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一株耐盐微生物的分离鉴定及其对养殖尾水降解效果研究
Isolation and Identification of A Salt-Tolerant Microorganism and Its Degradation Effect to Cultured Tail Water
【摘要】 随着近海养殖业的迅猛发展,高密度养殖尾水的直接排放对海洋环境的破坏日趋严重,有效地处理养殖尾水已成为当务之急。微生物修复技术因兼具高效性和环保性而成为尾水处理的首选。本研究从高密度养殖尾水中分离纯化出1株耐盐微生物,通过形态学观察、生理生化反应和16S rDNA基因测序方法鉴定该菌株属于交替假单胞菌属。对该菌的降解能力初步研究显示,该菌以1:100的接种比例加入凡纳滨对虾高密度养殖尾水后,在28℃、200 r·min-1的条件下反应7 d,其对养殖尾水中的氨氮及COD具有一定降解作用,但对总磷的降解效果不明显。
【Abstract】 With the rapid development of the offshore aquaculture industry, the discharge of high-density breeding tail water to the marine environment is becoming more and more serious, and effective treatment of breeding tail water has become a top priority. Microbial remediation technology has become the first choice for tail water treatment due to its high efficiency and environmentally friendly. In this study, a salt-tolerant microorganism was isolated and purified from high-density farming tail water. The strain was identified as Pseudoalteromonas sp. by morphological observation, physiological and biochemical reactions and 16 S rRNA gene sequencing. A preliminary study on the degradation effects of this bacterium showed that the bacterium was added to the high-density culture tail water of Litopenaeus vannamei at a 1:100 inoculation ratios, and cultured at 28 ℃, 200 r·min-1 for 7 days, the ammonia nitrogen and COD in the water have a good degradation effect, but the degradation effect on the total phosphorus is not so obvious.
【Key words】 salt-tolerant bacteria; identification; breeding tail water; degradation effect;
- 【文献出处】 浙江海洋大学学报(自然科学版) ,Journal of Zhejiang Ocean University(Natural Science) , 编辑部邮箱 ,2021年02期
- 【分类号】X172;X714
- 【下载频次】194