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基于转录组测序的槟榔叶片黄化的共同差异表达基因的分析

Analysis of Common Differentially Expressed Genes in Leaf Yellowing of Areca Plam Based on Transcriptome Sequencing

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【作者】 禤哲; 车海彦; 曹学仁; 贺延恒; 罗大全;

【Author】 XUAN Zhe;CHE Haiyan;CAO Xueren;HE Yanheng;LUO Daquan;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Pests Comprehensive Governance for Tropical Crops, Ministry of Agriculture and Rural Affairs/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests;Guangdong Agribusiness Produce Quality Safety Testing Center Co., Ltd.;

【通讯作者】 罗大全;

【机构】 中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室; 广东广垦农产品质量安全检测中心有限公司;

【摘要】 槟榔(ArecacatechuL.)作为海南省重要的经济作物,其叶片黄化现象日趋严重,已成为当前制约槟榔产业持续健康发展的一大障碍。经田间调研发现,叶片黄化症状主要有从叶缘1/4处开始发生的特异性黄化和全叶型黄化两种类型。本研究应用转录组测序技术,筛选出440个在特异性叶片黄化组中共同显著差异表达的基因,这些差异基因主要富集在能量代谢通路、激素代谢通路和次级代谢通路中。本研究探讨了不同胁迫下槟榔叶片表现出特异性黄化症状的分子机理,为槟榔黄化病的综合防控工作提供理论依据。

【Abstract】 Areca catechu L. is an important economic crop in Hainan, China, the leaf yellowing has become increasingly serious, which is a major obstacle to the sustainable and healthy development of the areca industry. According to field research, the symptoms of leaf yellowing mainly included specific yellowing from 1/4 of leaf margin and whole leaf yellowing. In this study, 440 differentially co-expressed genes were screened in the specific leaf yellowing group by transcriptome sequencing, which were mainly concentrated in secondary metabolic pathways, hormone signal transduction pathways and energy metabolism pathways. This study explored the molecular mechanism of specific etiolation of areca leaves under different stresses and provided theoretical basis for the comprehensive prevention and control of areca plam yellowing disease.

【关键词】 槟榔; 黄化; 转录组测序;
【Key words】 Areca catechu L.; leaf yellowing; transcriptome sequencing;
【基金】 海南省重大科技计划项目(No.ZDKJ201817);中国热带农业科学院基本科研业务费专项(No.1630042017023)
  • 【文献出处】 热带作物学报 ,Chinese Journal of Tropical Crops , 编辑部邮箱 ,2021年10期
  • 【分类号】S763.7
  • 【下载频次】320
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