【摘要】 目的考察环介导等温扩增技术(LAMP)检测鼠疫实验感染动物的敏感性及检测时间,探讨在鼠疫流行现场应用LAMP技术的可行性。方法应用鼠疫耶尔森菌制备不同浓度的菌悬液感染各类实验动物,死亡后取肝脾进行LAMP扩增、细菌学培养、鼠疫耶尔森菌反向血凝试验(RIHA)、鼠疫胶体金免疫层析法(RGICA)检测。并就实验结果做相关分析判断。结果取实验感染动物材料做不同稀释度观察,LAMP检测滴度高于RIHA,具有较强的敏感性,能够快速检测。LAMP检出率高于RIHA及RGICA,与细菌培养的符合率为100%。结论 LAMP技术具有高度敏感性,1 h内肉眼可判读结果,在鼠疫流行现场应用LAMP技术具有可行性。更多还原

【Abstract】 Objective To explore the sensitivity and detection time of loop-mediated isothermal amplification(LAMP)technique in detecting experimentally infected animal plague, and to explore the feasibility of applying LAMP technique in plague.Methods Yersinia pestis was used to prepare different concentrations of bacteria suspension to infect various kinds of experimental animals.After the death of experimental animals, liver and spleen were tested by LAMP amplification, bacteriological culture, Yersinia pestis reverse hemagglutination test(RIHA)and plague colloidal gold immunochromatography(RGICA).Correlation analysis and judgment were made based on the experimental results.Results Different dilutions of experimental infected animal materials were observed in the experiment.The titer of LAMP was higher than that of RIHA,which was sensitive and rapid.LAMP detection rate was higher than that of RIHA and RGICA,and the coincidence rate with bacterial culture was 100%.Conclusion LAMP technique is highly sensitive, and the results can be read in 1 h.It is feasible to apply LAMP technique in plague.更多还原