【摘要】 建立环酯草醚液相色谱分析方法,为该产品质量控制提供技术支撑。采用高效液相色谱法,以乙腈+磷酸水溶液[55:45(体积比)]为流动相,使用ZORBAX SB-C18不锈钢柱和具有可变波长紫外检测器对环酯草醚原药、24.3%环酯草醚悬浮剂进行分离和定量分析。在229.83～839.48 mg/L的浓度范围内,环酯草醚原药和24.3%环酯草醚悬浮剂的浓度与其响应峰面积之间呈现出良好的线性相关,线性方程为y=5.31x-3.44,其相关系数为1.00;标准偏差分别为0.19、0.10;变异系数分别为0.19%、0.42%;24.3%环酯草醚悬浮剂平均回收率为100.76%。该方法操作简便、快速、准确,分离效果好,是环酯草醚较为理想的分析方法。更多还原

【Abstract】 This study was to provide technical support for pyriftalid quality control by establishing the HPLC analysis method. The method was developed for the separation and determination of pyriftalid TC、pyriftalid 24.3% SC, using acetonitrile + phosphoric acid aqueous solution [55∶45(volume ratio)] as mobile phase, by high performance liquid chromatography on ZORBAX SB-C18 column with variable wave length and UV detector. In the concentration range of 229.83～839.48 mg/L, the concentration of pyriftalid TC、pyriftalid 24.3% SC showed a fine linear correlation with the response peak area. The linear equation was y=5.31 x-3.44, the linear correlation coefficient was 1.00, the standard deviation were 0.19、0.10, the variation coefficient were 0.19%、0.42%, the average recovery was 100.76% respectively for pyriftalid TC and pyriftalid 24.3% SC. This method has the advantages of simple operation, rapidity, accuracy and well separation effect. It is a preferred analytical method for pyriftalid.更多还原