【摘要】 卵形蛋白(ovate family proteins, OFPs)基因家族是植物特有的转录因子之一,在植物响应低温胁迫中具有重要作用。为了探究小麦(Triticum aestivum)中的OFP家族成员及其低温响应特征,本研究在小麦全基因组范围内鉴定OFP基因家族成员并进行生物信息学分析,利用RNA-seq测序数据,分析了不同组织及低温胁迫下小麦TaOFPs的表达模式。结果显示,小麦基因组中共鉴定出100个TaOFPs,在A、B和D亚基因组均匀分布;系统发育分析将小麦、水稻(Oryza sativa)及拟南芥(Arabidopsis thaliana)的OFP基因分为6组,禾本科水稻及小麦OFP基因在进化上更为保守;启动子元件分析结果表明,TaOFP家族基因启动子含有大量茉莉酸甲酯响应元件、脱落酸响应元件、低温响应元件、干旱响应元件等激素和非生物胁迫响应元件;表达模式分析表明,TaOFP家族基因呈现组织和时期特异性表达;冷冻胁迫下,25个TaOFPs基因仅在冷驯化冷冻后呈现差异表达,且TaOFP4-1D,TaOFP10-2B,TaOFP27-4D,TaOFP31-5A和TaOFP33-5D基因在冷驯化冷冻和非冷驯化冷冻后呈现相反表达模式,推测这些基因参与小麦的抗寒性调控。本研究为进一步探究TaOFP基因家族成员的功能及解析其在低温胁迫响应中的调控作用提供参考依据。更多还原

【Abstract】 Ovate family proteins(OFPs) is a plant-specific transcription factor gene family, which plays an important role in low temperature stress response. In order to explore the OFP gene family members in wheat(Triticum aestivum), as well as the expression characteristics under cold stress, in this study,the members of the OFP family were identified and bioinformatics analysis was carried out in the whole genome of wheat, the expression pattern of TaOFPs in different tissues under low temperature stress were analyzed based on RNAseq. The results showed that a total of 100 Ta OFPs were identified, which were evenly distributed on A, B and D subgenomes. Wheat, rice(Oryza sativa) and Arabidopsis OFPs were divided into 6 subgroups based evolutionary analysis, and the OFP genes in gramineae were more conservative during evolution. The results of promoter elements analysis indicated that a large number of hormone related and abiotic stress response cisacting elements, such as MeJA responsive elements, ABA responsive elements, low temperature responsive elements and drought responsive elements existed in promoter sequences of TaOFPs. The expression analysis revealed the tissue and stage specific expression patterns of TaOFPs. Under freezing stress, 25 TaOFPs were differentially expressed in cold acclimation and freezing specificity, TaOFP4-1D, TaOFP10-2B, TaOFP27-4D,TaOFP31-5A and TaOFP33-5D showed opposite expressed pattern in cold acclimation and freezing and uncold acclimation and freezing treatments, suggested that these genes may involved in regulation of cold resistance of wheat. This study provides reference for further exploring the function of TaOFP family members and analyzing its regulatory mechanism in low-temperature stress.更多还原