【摘要】 胁迫相关蛋白(stress associated proteins, SAPs)是一类具有A20和/或AN1结构域的锌指蛋白,广泛参与植物对逆境胁迫的响应。本研究探讨小麦(Triticum aestivum) TaSAP2-6A基因标记与多种环境下农艺性状的相关性,为发掘利用优异等位变异提供依据。本研究克隆得到小麦TaSAP2-6A基因(GenBank No. JQ768347.1),其基因组序列全长3 409 bp,包括编码区上游序列2 714 bp,3’端非翻译区167 bp,以及编码区528 bp。该基因编码175个氨基酸,包含1个A20和1个AN1结构域。利用一套以’中国春’为背景的缺-四体系将该基因定位到小麦的染色体6A上。通过32份多态性较高的小麦材料检测TaSAP2-6A基因序列多态性,在编码区未发现核苷酸变异位点,在上游序列1 527 bp位点检测到1个插入-缺失(insertion-deletion, InDel)位点(T/-)。根据该位点设计衍生酶切扩增多态性序列(derived cleaved amplified polymorphic sequence, dCAPS)标记InDel-1527,检测由323份小麦材料组成的自然群体的基因型。标记InDel-1527与农艺性状的关联分析显示,在干旱和热等10种环境条件下,该标记与小麦株高、穗粒数及单株产量显著或极显著相关,当该位点为T时,是降低株高、增加穗粒数和单株产量的优异等位变异。本研究结果为小麦分子标记辅助育种提供了基因资源及其功能分子标记。更多还原

【Abstract】 Stress associated proteins(SAPs) are the A20/AN1 zinc-finger proteins which widely involved in plant response to abiotic stress. The present study investigated the correlation between TaSAP2-6 A gene marker and agronomic traits in a variety of environments to provide a basis for the exploration and utilization of excellent allelic variation in wheat(Triticum aestivum). In this study, TaSAP2-6 A gene(GenBank No.JQ768347.1) was isolated from wheat, with the full-length genome sequence of 3 409 bp including the upstream sequence(2 714 bp), 3’ untranslated region(167 bp) and coding region(528 bp). The coding region encoded 175 amino acids consisting of an A20 domain and an AN1 domain. Using a set of ’Chinese Spring’nullisomic-tetrasomic lines, TaSAP2-6 A gene was located on chromosome 6 A. It was consistent with the predictions made from URGI(Unité de Recherche Génomique Info) website. The polymorphism of TaSAP2-6 A gene sequence was detected by 32 wheat accessions with high diversity, and no nucleotide variation was detected in the coding region, but one InDel(insert-deletion, T/-) site was identified at 1 527 bp site of the upstream sequence. A dCAPS(derived cleaved amplified polymorphic sequence) marker was developed based on the InDel, named as InDel-1527. The genotypes of a natural population consisted of 323 accessions were detected by the marker InDel-1527 of TaSAP2-6 A. Association analysis revealed that this marker was significantly associated with plant height, grain number per spike and grain yield per plant in ten environments(year×site×treatment) with well-watered, drought-stressed, heat-stressed, and drought-heat-stressed treatments.The InDel(T) was considered as the superior allelic variation to decrease plant height, and increase grain number per spike and grain yield per plant. The results provide a gene resource and its functional marker for marker-assisted selection in wheat.更多还原