【摘要】 目的:探讨γ-氨基丁酸(GABA)抑制腹主动脉瘤小鼠未成熟树突状细胞(iDCs)的迁移机制。方法:在腹主动脉瘤小鼠iDCs中转染GABA-A受体α5亚基SiRNA 48 h后,Western blot检测GABA-A受体α5亚基表达水平的变化。然后Transwell迁移实验检测GABA/GABA-A受体α5亚基对iDCs迁移能力的调控。激光共聚焦显微镜观察GABA/GABA-A受体α5亚基对iDCs伪足生成的影响。结果:在iDCs中转染GABA-A受体α5亚基SiRNA 48 h后,α5亚基的表达水平下降,差异有统计学意义(P<0.05)。GABA抑制iDCs的迁移,差异有统计学意义(P<0.05)。GABA抑制了iDCs伪足的形成。结论:GABA可能作用于GABA-A受体α5亚基抑制腹主动脉瘤小鼠iDCs的迁移和iDCs伪足的形成。更多还原

【Abstract】 Objective:To investigate the mechanism of GABA inhibiting migration function of immature dendritic cell(iDCs) in abdominal aortic aneurysm mice.Methods:48 hours after transfection of GABA-A receptor α5 subunit SiRNA in iDCs,the expression of GABA-A receptor α5 subunit was detected by Western blot.Then, the transwell migration experiment was performed to assess the regulation of iDCs migration ability by GABA/GABA-A receptor pathway.The regulatory effects of GABA/GABA-A receptor pathway on iDCs pseudopodia production was further analyzed by laser confocal microscopy.Results:48 hours after the transfection of GABA-A receptor α5 subunit SiRNA in iDCs, the expression of α5 subunit significantly decreased(P<0.05).The migration of iDCs was significantly inhibited by GABA(P<0.05).GABA inhibited the formation of iDCs pseudofoot.Conclusion: GABA/GABA-A receptor α5 subunit inhibits the migration ability of iDCs and the formation of iDCs pseudofoot.更多还原