【摘要】 目的:探究透皮活血中药垫枕枕芯对试验犬下肢深静脉血栓模型的安全性及有效性。方法:构建深静脉血栓比格犬模型,并随机分为实验组(透皮活血中药)和空白对照组,每组8只,分别于治疗前和治疗后1、2、4、12、16周抽血比较两组试验动物的D-二聚体、血常规、凝血功能及肝肾功等指标,并行彩超检查了解下肢血栓再通、复发情况;治疗后16周处死试验动物,取心、肝、肺、脑和肾脏标本行病理学检查。结果:两组试验动物进食正常、活动正常,血常规、凝血功能、肝肾功、电解质(血钾、钙、钠)在治疗后1、2、4、12、16周比较,差异无统计学意义(P>0.05);两组D-二聚体在治疗后1、2周比较,差异无统计学意义(P>0.05),但在治疗后4、12、16周,实验组的D-二聚体水平低于对照组,差异有统计学意义(P<0.05);治疗后16周内,两组心、肝、肺、肾、脑病理检查均未见异常;治疗后16周内,实验组的血栓复发率与对照组比较,差异无统计学意义(P>0.05),且实验组血栓完全再通,而对照组血栓部分再通。结论:透皮活血中药垫枕枕芯用于下肢深静脉血栓形成动物犬模型后,对血液常规、生化和重要脏器结构功能无明显影响,安全性较高,且可轻度降低血D-二聚体,降低血栓远期复发率,具有一定的临床应用前景及价值。更多还原

【Abstract】 Objective: To investigate the safety and effectiveness of lower limb deep venous thrombosis transdermal blood-activating traditional Chinese medicine bolster pillow inner through thrombosis models.Methods: Beagle dogs with deep vein thrombosis were randomly divided into experimental group(transdermal Chinese Medicine) and blank control group, 8 dogs in each group.Blood samples were collected to measure D-dimer, blood routine, blood coagulation function, liver and kidney functions, and electrolyte before treatment and 1,2,4,12 and 16 weeks after treatment.Colorful ultrasound dopplar were performed to investigate thrombus recanalization and reoccurrence.All animals were sacrificed in the 16 th week, and the tissue of heart, liver, lung, brain and kidney were subjected to pathological examination.Results: The animals in the two groups ate normally and moved normally, there was no significant difference in blood routine, coagulation function, liver and kidney function and electrolytes(blood potassium, calcium and sodium) between the two groups at 1,2,4,12 and 16 weeks after treatment(P>0.05).There was no significant difference in D-dimer between the two groups at 1 and 2 weeks after treatment(P>0.05),the plasma levels of D-dimer in the experimental group was lower than that in the control group at 4,12 and 16 weeks(P<0.05).Within 16 weeks after treatment, no abnormality was found in the heart, liver, lung, kidney and brain of the two groups.The recurrence rate of thrombosis in the experimental group was lower than that in the control group(0.00% vs.25.00%),the difference was not statistically significant(P>0.05).Thrombus of the experimental groups was all completely recanalized, and the thrombus of the control group was partially recanalized.Conclusion:The application of lower limb deep venous thrombosis transdermal blood-activating traditional Chinese medicine bolster pillow inner has no obvious effect on blood routine, biochemistry and important organ structure and function, it has high safety, and can slightly reduce blood D-dimer and reduce the long-term recurrence rate of thrombosis.It has a certain clinical application prospect and value.更多还原