【摘要】 [目的]探究福美双对肉鸡股骨生长发育的影响,评价巴戟天多糖（Morinda officinalis polysaccharide,MOP）对福美双诱导的肉鸡股骨损伤的保护效果。[方法]将300只1日龄AA肉鸡随机分为对照组、福美双组以及低、中、高剂量MOP组,每组3个重复,每个重复20只鸡。对照组全程饲喂基础饲粮;试验第4～7天,福美双组和各MOP组在基础饲粮中添加50 mg/kg福美双;在整个试验期内,低、中、高剂量MOP组分别在饮水中添加250、500、1 000 mg/L MOP。试验期为15 d。观察各组肉鸡临床表现;在试验第7、11、15天进行股骨和血液样品采集,对股骨进行形态学观察,测定股骨剪切力、物理指标（重量、长度、体积、密度）、干物质含量、钙磷含量、灰分_（DM）、灰分_（FM）、灰分密度,检测血浆钙离子和无机磷含量。利用统计学方法对各组试验数据进行比较分析。[结果]福美双组肉鸡出现跛行,严重者无法站立,股骨明显比对照组肉鸡短。试验第7天,与对照组相比福美双组股骨剪切力显著（P<0.05）降低。在整个试验期间,福美双组股骨的4项物理指标均显著（P<0.05）低于对照组;试验第11天低剂量MOP组的股骨重量显著（P<0.05）高于福美双组,试验第15天低剂量MOP组和中剂量MOP组的股骨长度显著（P<0.05）高于福美双组。试验第7天,福美双组股骨钙含量显著（P<0.05）低于对照组;试验第11天,福美双组股骨干物质含量、灰分_FM、灰分密度显著（P<0.05）低于对照组;试验第11天,高剂量MOP组与福美双组相比股骨干物质含量显著（P<0.05）增加。试验第15天中剂量MOP组的血浆无机磷含量与对照组相比显著（P<0.05）升高。[结论]福美双通过降低股骨强度、物理指标以及钙沉积,抑制肉鸡股骨生长发育;MOP对福美双诱导的肉鸡股骨损伤具有一定的保护和缓解效果。更多还原

【Abstract】 Objective]To assess the effect of thiram on femoral growth and development in broiler chickens,and to evaluate the protective efficacy of Morinda officinalis polysaccharide （MOP） against the femoral injuries induced by thiram.[Method]A total of 300 one-day-old AA broiler chickens were randomly divided into control group,thiram model group,and low-,moderate-,high-dosage MOP treatment groups.There were 3 replicates for each group and 20 broiler chickens for each replicate.The control group was fed with a basal diet;from 4th to 7^th days of the experiment,thiram model group and three MOP treatment groups were fed with basal diets added with 50 mg/kg thiram;during the whole experiment,250,500 and 1 000 mg/L MOP were supplied for low-,moderate-and high-dosage MOP treatment groups in drinking water.The experiment lasted for 15 days.The clinical manifestations of the broiler chickens in each group were observed;on 7^th ,11^th and 15thdays of the experiment,the femur and blood samples were collected;the morphological observation of femur was carried out,the shearing force,physical indexes to stand,and had shorter femur compared with those in control group.On 7^th day of the experiment,the femur shearing force of thiram model group was significantly （P<0.05） decreased compared with control group.During the whole experiment,the 4physical indexes of thiram model group were significantly （P<0.05） lower than those of control group;the femur weights of lowdosage MOP treatment group were significantly （P<0.05） higher than those of thiram model group on 11^th day of the experiment,and the femur lengths of low-and moderate-dosage MOP treatment groups were significantly （P<0.05） higher than those of thiram model group on 15thday of the experiment.The calcium contents in femur of thiram model group were significantly （P<0.05） lower than those of control group on 7^th day of the experiment,and the dry matter contents,ash contents（FM）,and ash densities in femur of thiram model group were significantly （P<0.05） lower than those of control group on 11^th day of the experiment;the dry matter contents in femur of high-dosage MOP treatment group were significantly （P<0.05） higher than those of thiram model group on 11^th day of the experiment.The plasma contents of inorganic phosphorus of moderate-dosage MOP treatment group were significantly （P<0.05） higher than those of control group on 15thday of the experiment.[Conclusion]Thiram inhibits the femoral growth and development in broiler chickens via decreasing the strength,physical indexes and calcium deposition of femur,and MOP can protect and alleviate the femoral injuries induced by thiram.更多还原