【摘要】 【目的】组配低温高效玉米秸秆降解复合菌,为玉米秸秆原位促腐还田提供微生物菌种资源。【方法】在低温15℃条件下,以玉米秸秆降解菌A3(Achromobacter deleyi strain LMG 3458)、A4(Pseudomonas plecoglossicida strain NBRC)组合为基础,通过添加功能真菌A(Aspergillus terreus)和P(Phanerochaete chrysosporium),比较复配菌A3+A4+A、A3+A4+P和A3+A4+A+P酶活性及其对玉米秸秆降解率和单菌微生物相对含量的影响。【结果】复配菌A3+A4中添加真菌A和P,其微生物相对含量增加,可显著提高玉米秸秆降解率(P<0.05)。复配菌A3+A4+A+P玉米秸秆降解率最高,为38.79%,较A3+A4玉米秸秆降解率提高了13.04个百分点。滤纸酶活性、木聚糖酶活性、漆酶活性分别在培养30、15、20 d时达到最高值,与A3+A4相比,分别提高了4.44 U/mL、4.00 U/mL、60.04 U/L。纤维素、半纤维素和木质素降解率较A3+A4分别提高了10.77、4.15和21.10个百分点。【结论】复配菌A3+A4+A+P具有较高的玉米秸秆降解效率,可为玉米秸秆微生物降解菌剂的研究及利用提供菌种资源。更多还原

【Abstract】 【Objective】To combine the high-efficient compound microbes that degrade corn straw at low temperature,and to provide candidate microbial strains for corn straw returned into field in situ under promoting decay condition. 【Methods】Based on the combination of corn straw degradation bacteria A3(Achromobacter deleyi strain LMG 3458)and A4(Pseudomonas plecoglossica strain NBRC),three sets of compound microbes were prepared by supplementing functional fungi A(Aspergillus terreus)and P(Phanerochaete chrysosporium),which were A3+A4+A,A3+A4+P,and A3+A4+A+P,respectively. The enzyme activities of the compound microbes as well as their effects on degradation rate of corn straw and relative microbial contents of single strain were studied at a low temperature of 15 ℃.【Results】 When fungi A and P were added,the relative microbial content of the bacterial combination of A3+A4 increased,and the corn straw degradation rate increased significantly(P<0.05).The compound microbes A3+A4+A+P had the highest corn straw degradation rate of 38.79%,which was 13.04 percentage points higher than that of the bacterial combination of A3 +A4. The highest FPase activity,xylanase activity,and laccase activity of the compound microbes A3+A4+A+P was observed on 30,15,and 20 day of incubation,respectively,which were4.44 U/mL,4.00 U/mL and 60.04 U/L higher than those of the bacterial combination of A3+A4,respectively. Furthermore,the degradation rates of cellulose,hemicellulose,and lignin of the compound microbes A3+A4+A+P were higher than those of the A3 +A4 by 10.77,4.15,and 21.10 percentage points,respectively. 【Conclusion】The compound microbes A3 +A4 +A +P had higher corn straw degradation efficiency and could be used as candidate strain resources for the study and application of microbial agents for corn straw degradation.更多还原