【摘要】 目的:探究小鼠肠上皮11β-羟类固醇脱氢酶(11β-hydroxysteroid dehydrogenase,11β-HSD1)基因特异性敲除对小肠上皮屏障功能的影响。方法:在C57BL/6J小鼠构建肠上皮特异性11β-HSD1敲除模型,野生型对照组和11β-HSD1敲除组分别给予高脂饮食喂养(第6周开始,喂养8周)。通过免疫组化方法观察小鼠小肠上皮绒毛、隐窝、杯状细胞数量、紧密连接蛋白-1(zona occludens-1,ZO-1)和炎症标志物F4/80的变化。结果:11β-HSD1基因敲除能够改变高脂饮食肥胖小鼠的绒毛长度和杯状细胞数量,显著减轻高脂饮食肥胖小鼠的小肠上皮炎症,改变紧密连接蛋白表达。结论:高脂饮食导致的小鼠小肠上皮屏障功能变化与11β-HSD1的作用密切相关。更多还原

【Abstract】 Objective:This study aims to explore the effect of 11β-hydroxysteroid dehydrogenase(11β-HSD1)gene specific knockout on intestinal epithelial barrier function in mice. Methods:the 11β-HSD1 knockout model was established in C57 BL/6 J mice.The wild-type control group and 11β-HSD1 knockout group were fed with high-fat diet(from the 6 th week,feed for 8 weeks). The number of villi,crypts,goblet cells,and expression changes of ZO-1 and F4/80 were detected by immunohistochemistry. Results:11β-HSD1 gene knockout could change the villus length and goblet cell number of obese mice fed with high-fat diet,significantly reduce the intestinal epithelial inflammation and change the expression of tight junction protein in obese mice fed with high-fat diet.Conclusion:The changes of intestinal epithelial barrier function induced by high-fat diet are closely related to the effect of 11β-HSD1.更多还原