【摘要】 通过将营口污水处理厂二沉池的泥水混合物进行静置沉淀,取上清液进行稀释筛选得到一株革兰氏阴性菌D-12。然后采用生物反应器内的营养液（Na OH+驯化液）对沼气中的H₂S进行洗脱,再采用筛选菌株D-12对含有H₂S的营养液进行反硝化脱硫（试验周期为38 d）。以初始S^2-浓度、微生物添加量和NO₃^-浓度为自变量,以降解试验中的S^2-去除率为因变量,采用响应曲面法Box-Behnken设计实验,研究各自变量及其交互作用对S^2-去除率的影响,并得到二次多项式回归方程预测模型。研究结果表明:NO₃^-在反应体系中可以作为有效电子受体,促进微生物将S^2-氧化为单质硫;优化后的试验条件为S^2-浓度为1 258.61 mg/L,NO₃^-浓度为843.96mg/L,微生物添加量为219.96 mg/L,此时S^2-去除率为98.38%,与S^2-预测去除率的相对误差为1.62%。更多还原

【Abstract】 A gram negative bacterium D-12 was obtained by static precipitation of dilution and screening of the supernatan the mud water mixture in the secondary sedimentation tank of Yingkou sewage treatment plant.Firstly,the first stage nutrient solution （Na OH+acclimation solution） in the reactor was used to elute the H₂S in the biogas.In the second stage,denitrifying S^2-removal reaction was carried out by screening strain D-12 with test period was 38 d.Taking the initial S^2-concentration,microbial biomass and nitrate as independent variables,and removal rate of S^2-in degradation experiment as dependent variables,the Box-Behnken design was used to study the effects of each variable and their interaction on S^2-removal,and a quadratic polynomial regression equation prediction model was obtained.The results showed that NO₃^-could be used as an effective electron acceptor in the reaction system to promote the oxidation of S^2-to elemental sulfur.When the optimal experimental conditions were:S^2-concentration of 1 258.61 mg/L,NO₃^-concentration of 843.96 mg/L and biomass of 219.96 mg/L,the average maximum removal rate of S^2-was 98.38%,and the relative error between the experimental results and the predicted value（removal rate） was 1.62%.更多还原