【摘要】 目的:建立消瘀跌打药酒制剂和其主药通城虎药材中马兜铃酸Ⅰ的含量测定方法。方法:采用高效液相色谱法。选用Inertsil ODS-XP （4.6 mm×150 mm,5μm）色谱柱,甲醇-0.05%冰醋酸溶液（53∶47）为流动相洗脱,检测波长:250 nm,流速:1 m L·min-1,柱温:30℃。结果:马兜铃酸Ⅰ的进样浓度在3.884～38.84μg·m L^-1范围内与峰面积积分值呈良好的线性关系,回归方程Y=55825X-12839 （r=0.9999）;消瘀跌打药酒中马兜铃酸Ⅰ的回收率平均为99.08%,RSD为0.61%;通城虎中马兜铃酸Ⅰ的回收率平均为98.65%,RSD为1.58%。结论:建立的消瘀跌打药酒制剂和通城虎药材中马兜铃酸Ⅰ含量测定方法,简便,重现性良好,可用于药材与制剂的质量控制。更多还原

【Abstract】 Objcetivet To establish the method for the content determination of aristolochic acid Ⅰ in Xiaoyudieda medicinal liquor and Aristolochia fordiana Hemsl. Methods HPLC method. Chromatographic conditions were as follows: Inertsil ODS-XP（ 4. 6 mm× 150 mm,5 μm） column was used with mobile phase consisted of methanol-0. 05% glacial acetic acid（ 53∶ 47） at a flow rate of1. 0 m L·min^-1 under a detection wavelength at 250 nm. The column temperature was 30 ℃ ． Results The linear range of aristolochic acid Ⅰ was 3. 884 ～ 38. 84 μg·m L-1,and the regression equation was Y = 55825 X-12839（ r = 0. 9999）. The average recovery rate of aristolochic acid Ⅰ in Xiaoyudieda medicinal liquor was 99. 08%,RSD was 0. 61%. The average recovery rate of aristolochic acid Ⅰ in Aristolochia fordiana Hemsl was 98. 65%,RSD was 1. 58%. Conclusion The method established in the experiment can be used to determine the content of aristolochic acid Ⅰ in Xiaoyudieda medicinal liquor and Aristolochia fordiana Hemsl. There were significant differences in aristolochic acid Ⅰ content between different producing areas and different batches.更多还原