【摘要】 【目的】组蛋白去乙酰化酶（Histone deacetylases,HDAC）在植物发育及抗病应答中发挥重要作用，本研究旨在鉴定棉花HDAC基因家族成员并对其在黄萎病抗性中的作用进行分析。【方法】利用生物信息学方法对棉花基因组中HDAC基因家族成员进行鉴定，并对其理化性质、系统发育关系、基因结构进行系统分析。利用实时荧光定量聚合酶链式反应分析了黄萎病菌诱导下瑟伯氏棉HADC基因的表达模式。【结果】陆地棉、亚洲棉和瑟伯氏棉基因组中分别包括30、15和13个HDAC基因。棉花HDAC分为RPD3/HDA1、HD2和SIR2三个亚族，同一亚族成员间具有相似的基因结构和保守基序。棉花HDACs基因启动子中含有大量的激素应答、胁迫响应以及植物生长发育相关的顺式作用元件。瑟伯氏棉GthHDAC基因响应乙烯、水杨酸和茉莉酸的诱导；在黄萎病菌胁迫下，多数GthHDAC基因上调表达，表明该基因家族可能通过乙烯/茉莉酸和水杨酸等信号通路调控棉花黄萎病抗性。【结论】本研究从全基因组水平上鉴定了亚洲棉、陆地棉和瑟伯氏棉中58个HDAC家族基因，并明确了瑟伯氏棉HDAC家族成员在黄萎病菌胁迫下的表达模式。黄萎病菌处理后，大部分Gth HDAC基因在叶片中上调表达，并且受到乙烯、水杨酸和茉莉酸的诱导。更多还原

【Abstract】 [Objective] Histone deacetylases(HDAC) plays an important role in plant development and disease resistance. Here,we intend to identify the members of HDAC gene family and its function in Verticillium wilt resistance of cotton. [Method] In this study, HDAC genes were identified and their physical and chemical properties, phylogenetic relationships, gene structures were analyzed by bioinformatics analysis. The expression patterns of HDAC genes under Verticillium dahliae stress were tested by qRT-PCR(Quantitative real-time polymerase chain reaction). [Result] A total of 30, 15, and 13 HDAC genes were identified in Gossypium hirsutum, G. arboreum, and G. thurberi, respectively. These genes were divided into RPD3/HDA1, HD2 and SIR2groups and possessed similar structures and conserved motifs in each group. A large number of cis-acting elements related to hormone response, stress response and plant growth and development were identified in the promoters of HDAC genes. The results also showed that HDAC genes could be induced by ethylene(ET), salicylic acid(SA) and jasmonic acid(JA) and up-regulated under Verticillium dahliae infection, which indicated HDAC genes probably involved in Verticillium wilt resistance by ET/JA and/or SA signding pathways in cotton. [Conclusion] In this study, a total of 58 HDAC genes were identified. Under the treatment of wilt, most of Gth HDAC genes were up-regulated in the leaves of G. thurberi, and some GthHDAC genes were induced by ET, SA and JA. These results proved that HDAC can be used in the future research to understand the molecular mechanism of Verticillium wilt tolerance in cotton.更多还原