【摘要】 【目的】为建立海岛棉发育过程中花蕾长度与花药发育时期的关系。【方法】以海岛棉新海35为研究对象,采用改良的半薄切片和甲苯胺蓝染色技术,对不同生长时期花蕾的花药进行切片观察,明确花蕾长度与花药发育时期的对应关系。【结果】通过形态观察发现,在海岛棉的花发育过程不同时期花蕾的萼片、花瓣和花药的形态、颜色、大小以及花丝和柱头的长度出现明显变化。切片观察发现,从花芽出现到花药开裂,经历花原基的形成、花药原基的出现、花粉母细胞形成、四分体时期、绒毡层降解、减数分裂、花药开裂等14个发育时期。新海35花蕾长度5～6 mm、7～8 mm、8～9 mm和11～14 mm分别对应花药发育中的花粉母细胞形成期、四分体时期、小孢子释放期和绒毡层降解时期,也是成花过程中尤为关键的4个时期。通过对花粉萌发、花粉管伸长、受精过程、胚乳的发育等进行观察,明晰了海岛棉花粉成熟到完成受精再到纤维发育所需的具体时间和详细过程。【结论】海岛棉各个时期花蕾的表型与陆地棉不同,且花蕾大小及花药颜色随着花药细胞发育而变化,并伴随着细胞内部的代谢过程及正常发育所需物质的转换;通过花蕾大小及表型可以判定海岛棉花药发育时期。花粉成熟后,花粉的萌发、花粉管的伸长、双受精及胚乳发育分别发生在授粉后的0.5～1 h、8 h、24 h和50 h。更多还原

【Abstract】 [Objective] The study aims to establish the relationship between flower bud length and anther development in sea-island cotton. [Method] Anther development with different lengths of flower buds in Gossypium barbadense L. Xinhai 35 were observed by semi-thin section and toluidine blue staining. [Results] The color, shape, and size of sepals, petals, anthers,the lengths of filaments and stigmas changed with the development of flower buds in sea-island cotton. From flower buds development to anther dehiscence, the process have 14 stages including flower primordia formation, anther primordia occurrence, microspore mother cells formation, tetrad stage, tapetum degradation, meiosis, and anther dehiscence. At the pollen mother cell formation stage, tetrad stage, the stage of microspore release, and tapetum degradation, the bud length are 5-6 mm,7-8 mm, 8-9 mm and 11-14 mm of Gossypium barbadense L. Xinhai 35, respectively. They are four key stages in the process of flowering. The whole process of fertilization including pollen germination, pollen tube elongation, fertilization and endosperm development were observed and clarified. [Conclusion] The morphology of flower bud at different stages in sea-island cotton are different from upland cotton. The size of flower bud and the color changed with the development of anther cells. At the same time, metabolic process happened and some substances were transported in normal cell development. According to the morphology of flower bud, sea-island cotton developing periods can be determined. After pollen maturation, pollen germination,pollen tube elongation, double fertilization and endosperm development occurred at 0.5-1 h, 8 h, 24 h and 50 h after pollination,respectively.更多还原