【摘要】 目的观察氢吗啡酮预处理对大鼠肾缺血再灌注损伤时PI3K/Akt的影响。方法 36只SD大鼠随机分为3组:假手术组(S组)、缺血再灌注组(IRI组)和氢吗啡酮预处理组(H组),每组12只。H组大鼠于造模前25 min腹腔注射氢吗啡酮30μg/kg, S组和IRI组注射等量生理盐水,采用夹闭双侧肾动脉制备缺血再灌注模型。HE染色观察肾组织病理改变;ELISA法检测血BUN、Scr、TNF-α、IL-6、MDA、SOD水平;Western blot法检测肾组织Bax、Bcl-2、p-PI3K、p-Akt蛋白表达。结果与S组比较,IRI组和H组BUN、Scr、MDA、TNF-α、IL-6、Bcl-2、p-PI3K、p-Akt含量均明显升高,SOD、Bax均明显降低(P<0.05),肾病理损伤明显;与IRI组比较,H组BUN、Scr、MDA、TNF-α、IL-6、Bcl-2含量均明显降低,SOD、Bax、p-PI3K、p-Akt含量均明显升高(P<0.05),肾病理损伤减轻。结论氢吗啡酮可通过激活PI3K/Akt信号通路,调控氧化应激、炎症反应、细胞凋亡水平,减轻大鼠肾缺血再灌注损伤。更多还原

【Abstract】 Objective To investigate the effect of hydromorphone pretreatment on phosphatidylinositol 3-kinase/protein kinase B(PI3 K/Akt) of renal ischemia-reperfusion injury in rats.Methods Thirty-six SD rats were randomly divided into sham-operation group(group S),ischemia-reperfusion group(group IRI) and hydromorphone pretreatment group(group H),with 12 rats in each group.Rats in group H were intraperitoneally injected with hydromorphone 30 μg/kg at 25 min before modeling, while rats in group S and group IRI were intraperitoneally injected with equal volume of normal saline.Renal ischemia-reperfusion injury model was established by clipping bilateral renal artery.The pathological changes of renal tissues were observed by HE staining; the level of BUN,Scr, TNF-α,IL-6,MDA and SOD in serum was detected by ELISA;the expression of Bax, Bcl-2,p-PI3 K and p-Akt protein was detected by Western blot.Results Compared with group S,the contents of BUN,Scr, MDA,TNF-α,IL-6,Bcl-2,p-PI3 K and p-Akt in group IRI and group H were significantly increased, while the levels of SOD and Bax were significantly reduced(P<0.05),the pathological damage of renal tissues being obvious; compared with group IRI,the levels of BUN,Scr, MDA,TNF-α,IL-6 and Bcl-2 in group H were significantly reduced, while the contents of SOD,Bax, p-PI3 K and p-Akt were significantly increased(P<0.05),the pathological damage of renal tissues being relieved.Conclusion Hydromorphone can regulate oxidative stress, inflammatory response and cell apoptosis by activating PI3 K/Akt signaling pathway, and alleviate the renal ischemia-reperfusion injury in rats.更多还原