【摘要】 目的探讨不同抗原格局抗筛细胞联合应用的意义,避免不规则抗体漏检。方法选取1 200例拟输血患者血浆标本,先用抗人球蛋白检测卡进行不抗规则体筛查,实验结果阳性标本用谱细胞鉴定抗体特异性;实验结果阴性标本分别再用不同实验方法和不同筛选细胞进行检测。结果不规则抗体筛查总阳性率为0.75%(9/1 200),其中使用单一试剂检出阳性率为0.58%(7/1 200),对其余抗筛结果阴性标本选用两种不同抗原格局筛选细胞检出抗体阳性2例,漏检率为0.17%(2/1 193)。结论应用木瓜酶法可以提高抗体检出率,不同抗原格局抗筛细胞联合应用可以有效预防不规则抗体漏检,降低输血不良反应的发生。更多还原

【Abstract】 Objective To explore the significance of combined application of screening cells with different antigen patterns to avoid missed detection of irregular antibodies.Methods Plasma specimens of 1 200 patients undergoing blood transfusion were selected.Gel test was used for irregular antibodies.Positive specimen was further evaluated for identification of specific alloantibody with cell panel.Negative specimen was tested by different methods and screening cells.Results The total positive rate of irregular antibodies was 0.75% (9/1 200),of which the positive rate tested by a single reagent was 0.58% (7/1 200).Two different antigen patterns were used to screen the remaining negative samples,two samples were positive and the missed detection rate was 0.17% (2 /1 193).Conclusion The use of papain enzymatic method can increase the positive rate of alloantibodies detection.The combined application of screening cells with different antigen patterns can effectively prevent the missed detection of irregular antibodies and reduce the occurrence of transfusion reactions.更多还原