【摘要】 目的探究SALL4基因与KLF基因在肝内胆管癌中的相互作用与影响。方法采用生物信息学方法预测SALL4与KLF4基因启动子之间的作用位点,并通过荧光素酶报告系统进行验证;RNA干扰技术对SALL4基因进行敲除,Western blotting检测KLF4蛋白的表达;免疫组化法检测临床样本中SALL4与KLF4蛋白的表达,并结合TCGA数据库进行验证。结果 KLF4启动子上存在SALL4结合的模序,且二者的表达呈负相关。结论在肝内胆管癌中,SALL4能够结合KLF4启动子区域,抑制KLF4基因的表达。在肝内胆管癌细胞株中敲低SALL4基因,可使E-cadherin表达显著提高,提示SALL4能够促进上皮间质转化(EMT)的改变。更多还原

【Abstract】 Objective To explore the interaction and effects of SALL4 gene and KLF gene in intrahepatic cholangiocarcinoma.Methods The interaction site between SALL4 and KLF4 gene promoter was predicted by bioinformatics method and verified by luciferase reporter system.SALL4 gene was knocked out by RNA interference,and KLF4 protein expression was detected by Western blotting.The expression of SALL4 and KLF4 proteins in clinical samples was detected by immunohistochemistry,and verified by TCGA database.Results There were SALL4 binding motifs on the KLF4 promoter.And there was a negative correlation between the expressions of SALL4 and KLF4 in vivo and in vitro.Conclusion SALL4 can bind to KLF4 promoter region and inhibit KLF4 gene expression in intrahepatic cholangiocarcinoma.Knockdown of SALL4 increased the expression of E-cadherin,suggesting that SALL4 could promote epithelial-mesenchymal transition.更多还原