【摘要】 目的：以红细胞膜为载体制备藤黄酸仿生纳米粒(GPP@RBC-NPs)，并考察其体外释放特性。方法：采用薄膜分散法制备GPP@RBC-NPs的中间产物GPP-NPs，以粒径、多分散指数(PDI)和包封率为评价指标，星点设计-效应面法优选GPP-NPs的制备工艺，再通过挤出法用RBCm包裹，得到GPP@RBC-NPs，对最优处方工艺制备的GPP@RBC-NPs进行理化性质和体外释放特性的考察。结果：GPP@RBC-NPs最佳工艺条件为：药载比为1:10；生理盐水与PEG3400-PLA2000用量的比为1.7:10(mL:mg)；探头超声时间和功率分别为10min和10%;RBC膜的稀释倍数及破碎时间分别为4倍和3 min。GPP@RBC-NPs平均粒径为（94.10±1.67）nm,Zeta电位为（-6.96±0.60）mV，包封率为(79.11±1.42)%。透射电镜扫描结果表明，GPP@RBC-NPs呈粒径均匀的球形。稳定性结果表明，GPP@RBC-NPs在4℃条件下，储存稳定性良好。体外释放结果表明，72 h内GPP@RBC-NPs累计释放量30.35%，能明显延长GA的释放时间。结论：GPP@RBCNPs制备工艺简单，可重复性较好，明显改善了GA的溶解性和体外释放性能。更多还原

【Abstract】 Objective: To prepare gambogic acid bionic nanoparticles(GPP@RBC-NPs) using erythrocyte membrane as carrier and to investigate its release characteristics in vitro. Method: The intermediate product GPP-NPS of GPP@RBC-NPs was prepared by thin-film dispersion method. The particle size, polydispersion index(PDI) and encapsulation rate were used as evaluation indexes. The preparation process of GPP-NPS was optimized by central design-response surface method. RBCm was used to coat the product by extruding, and GPP@RBC-NPs were obtained. The physicochemical properties and in vitro release characteristics of GPP@RBC-NPs prepared by the optimal formulation process were investigated. Result: The optimal technological conditions of GPP@RBC-NPs were drug loading ratio of 1:10. The dosage ratio of normal saline to PEG3400-PLA2000 was 1.7:10(mL: mg). The ultrasonic time and power of the probe were 10 min and 10%, respectively. The dilution ratio and crushing time of RBCm were 4 times and 3 min, respectively. The average particle size of GPP@RBC-NPs was(94.10±1.67) nm. The Zeta potential was(-6.96±0.60) mV, and the encapsulation rate was(79.11±1.42) %. The scanning results of transmission electron microscope showed that GPP@RBC-NPs were spherical with uniform particle size. The stability results showed that GPP@RBC-NPs had good storage stability at 4℃. The results of in vitro release showed that the cumulative release amount of GPP@RBC-NPs was 30.35% within 72 h, which could significantly prolong the release time of GA. Conclusion: The preparation process of GPP@RBC-NPs is simple and reproducible, and the solubility and in vitro release properties of GA are improved obviously.更多还原