【摘要】 目的:对长期咀嚼槟榔牙周炎人群与牙周健康人群牙龈组织中B淋巴细胞、效应B细胞及相关因子表达的差异性进行分析。方法:收集2020年11月～12月于中南大学口腔医学中心就诊的长期咀嚼槟榔牙周炎患者牙龈组织20例,以及同期在我院行牙冠延长术的健康人群牙龈组织20例。HE染色观察牙龈组织病理学形态,免疫组织化学染色检测对牙龈组织B淋巴细胞及效应B细胞(CD19、CD38、CD138)表达水平;ELISA法检测牙龈组织B细胞激活因子(B-cell activation factor, BAFF)及人可溶性RANK配体(soluble receptor activator of NF-κB ligand, sRANKL)水平。结果:长期咀嚼槟榔牙周炎人群的牙龈组织存在较多的炎性浸润,牙龈组织B淋巴细胞及效应B细胞(CD19、CD38、CD138)数量显著高于牙周健康人群(P<0.01);长期咀嚼槟榔牙周炎人群牙龈组织中BAFF及sRANKL表达水平显著高于牙周健康人群(P<0.05,P<0.01)。结论:长期咀嚼槟榔牙周炎人群牙龈中B淋巴细胞、效应B细胞及相关因子BAFF、sRANKL表达均较健康人群牙龈组织上升,提示长期咀嚼槟榔人群发生牙周炎可能与B淋巴细胞及效应B细胞密切相关,槟榔可能在牙周炎发生发展的病理过程中扮演着重要角色。更多还原

【Abstract】 Objective:To analyze the difference of expression of B lymphocytes, effector B cells and related factors in gingival tissues between chronic chewing betel nut periodontitis and periodontal healthy people.Methods:The gingival tissue of 20 patients with chronic chewing betel nut periodontitis and 20 healthy people who underwent crown lengthening in our hospital during the same period were collected from November to December 2020.The pathological morphology of gingival tissue was observed by HE staining.The expression levels of B lymphocytes and effector B cells(CD19,CD38,CD138)in gingival tissue were detected by immunohistochemical staining.The levels of B-cell activation factor(BAFF)and soluble receptor activator of NF-κB ligand(sRANKL)in gingival tissue were detected by ELISA.Results:The number of B lymphocytes and effector B cells(CD19,CD38,CD138)in gingival tissue was significantly higher than that in periodontal healthy people(P<0.01).The expression levels of BAFF and SRANKL in gingival tissues of chronic chewing betel nut periodontitis group were significantly higher than those of periodontal healthy group(P<0.05,P<0.01).Conclusion:The expressions of B lymphocytes, effector B cells and related factors BAFF and sRANKL in gingival tissues of people with periodontitis chewing betel nut for a long time are all higher than those of healthy people, suggesting that periodontitis in people who chewing betel nut for a long time may be closely related to B lymphocytes and effector B cells.Betel nut may play an important role in the pathological process of periodontitis.更多还原