【摘要】 目的探究中性粒细胞胞外诱捕网(neutrophil extracellular traps, NETs)对重度牙周炎患者凝血指标的影响。方法纳入20例重度牙周炎患者作为试验组,20例健康人作为对照组,记录牙周指标、凝血指标。ELISA测定外周血中NETs标志物cf-DNA和MPO-DNA的含量,免疫荧光显微镜观察不同来源中性粒细胞受到刺激后产生NETs的能力。分析NETs标志物与凝血指标之间的相关性。结果重度牙周炎组的牙周指标、NETs标志物、中性粒细胞的活性、凝血指标均显著高于对照组(P<0.05)。结论 NETs在重度牙周炎患者高凝状态的形成中发挥重要作用。更多还原

【Abstract】 Objective To explore the effect of neutrophil extracellular traps(NETs) on the coagulation indexes of severe periodontitis. Methods Twenty patients with severe periodontitis and 20 healthy people were included as the experimental and control group respectively. Periodontal indexes and coagulation indexes were recorded. The markers of cell free-DNA(cf-DNA) and myeloperoxidase-deoxyribonucleic acid(MPO-DNA) were measured by ELISA in peripheral blood. The ability of neutrophils from different sources to produce NETs after being stimulated was observed by immunofluorescence microscope. The correlation between NETs markers and coagulation indicators was analyzed. Results Periodontal indexes, NETs markers, neutrophil activity, and coagulation indexes in the severe periodontitis group were significantly higher than those in the control group(P<0.05). Conclusion NETs play an important role in the formation of hypercoagulable state in severe periodontitis patients.更多还原