【摘要】 目的构建M2e-HA2串联表位的自组装纳米颗粒通用流感疫苗,并进行免疫血清学检测。方法将流感病毒M2e和HA2抗原表位与Ferritin蛋白串联表达,非变性条件下提取并经亲和层析纯化获得纳米自组装颗粒,经Western blot和电镜验证后免疫小鼠。2次免疫后采集小鼠血清,使用Western blot进行抗原性检测,使用ELISA进行抗体水平检测。结果成功制备M2e-HA2串联表位的自组装纳米颗粒,将其免疫小鼠后血清中产生高水平的M2e和HA2特异性抗体。结论 M2e-HA2串联表位的自组装纳米颗粒可以刺激小鼠产生高水平的M2e和HA2特异性抗体,为通用流感疫苗研发奠定基础。更多还原

【Abstract】 Objective To construct the self-assembled nanoparticle influenza vaccine with the M2 e-HA2 epitopes, and detect the immunological serology. Methods The M2 e and HA2 antigen epitopes of influenza viruses were expressed in tandem with Ferritin protein,and the nanometer self-assembled particles were extracted under the condition of non-denatured and purified by affinity chromatography. The mice were immunized for two times,after the two immunizations,serum of mice was collected,western blot was used for antigenicity detection,and ELISA was used for antibody level detection. Results M2 e-HA2 self-assembled nanoparticles were successfully prepared, and high levels of M2 e and HA2 specific antibodies were produced in the serum of immunized mice. Conclusion M2 e-HA2 self-assembled nanoparticles can stimulate the production of high levels of M2 e and HA2 specific antibodies in mice,laying the foundation for the development of a universal influenza vaccine.更多还原