【摘要】 【目的】建立米蛾Corcyra cephalonica成虫转录组数据库,获得米蛾的嗅觉基因信息。【方法】采用高通量测序平台(Illumina Hiseq)对米蛾进行转录组测序,用Trinity软件对原始数据进行组装分析,在7个数据库中用Blast软件对unigene进行注释,用PCR方法检测米蛾信息素结合蛋白基因(Pheromone bindingprotein,PBPs)和普通气味结合蛋白基因(General odorant bindingprotein,GOBPs)在成虫不同组织中的表达情况。【结果】最终获得58 534条unigene,其中25 879条得到注释;N50为2 212 bp,平均长度1 251 bp;与脐橙螟Amyelois transitella相似度最高(28.2%);发掘了20个气味结合蛋白(Odorant binding proteins, OBPs),18个化学感受蛋白(Chemosensory proteins, CSPs),2个GOBPs和3个PBPs,均已上传NCBI。组织表达分析发现PBPs和GOBPs基因都在米蛾成虫触角上大量表达,在头、胸、腹、足、翅中少量表达,PBP3在成虫胸部组织中无表达,GOBP2仅在成虫触角上大量表达,在其他组织中无表达。【结论】研究结果丰富和完善了米蛾的嗅觉基因信息,为米蛾的嗅觉识别机制研究和诱剂研发奠定了分子基础。更多还原

【Abstract】 [Objectives] To obtain olfactory gene information and establish a transcriptome database for the adult rice moth,Corcyra cephalonica. [Methods] The C. cephalonica transcriptome was sequenced on the Illumina HiSeq platform, the original data were assembled and analyzed using the Trinity software package and Blast was used to annotate unigenes in 7 databases. The expression of pheromone binding proteins(PBPs) and general odorant binding proteins(GOBPs) in different adult moth tissues was then detected using PCR. [Results] A total of 58 534 unigenes were obtained, 25 879 of which were annotated with an N50 value of 2 212 bp and mean length of 1 251 bp. C. cephalonica unigenes had highest similarity(28.2%)to those of Amyelois transitella; 20 odorant binding proteins(OBPs) genes, 18 chemosensory proteins(CSPs) genes, 2 general odorant binding protein(GOBPs) genes and 3 pheromone binding proteins(PBPs) genes, were identified and uploaded to NCBI. PBPs and GOBPs were highly expressed in the antennae of adult moths but expression was lower in the head, thorax,abdomen, leg and wing. PBP3 was not expressed in the thorax, and GOBP2 was only highly expressed in the antennae and not in other tissues. [Conclusion] The results significantly increase the available information on the olfactory genes of C.cephalonica, and provide a molecular foundation for the study of olfactory recognition mechanisms and the development of attractants for this pest.更多还原