【摘要】 目的探讨结肠癌转移相关基因1(MACC1)对卵巢癌细胞生长与迁移的影响。方法体外培养人卵巢癌SKOV3细胞,实验分3组:正常对照组、阴性对照组和si-MACC1组;各组细胞转染48 h后,采用MTT法检测细胞增殖,Transwell小室检测细胞迁移,流式细胞仪检测细胞凋亡,western blot法检测各组细胞MACC1、PI3K、p-AKT、PCNA及cleaved caspase 3蛋白的表达。结果与正常对照组或阴性对照组比较,si-MACC1组SKOV3细胞增殖活性和迁移能力均降低(均P<0.05),而凋亡率增加(P<0.01);si-MACC1组细胞中MACC1、PI3K、p-AKT及PCNA蛋白水平较正常对照组及阴性对照组均显著降低(均P<0.05),而cleaved caspase 3蛋白则显著升高(P<0.05)。结论沉默MACC1基因可抑制人卵巢癌SKOV3细胞的生长和迁移,其机制可能是MACC1激活PI3K/AKT信号通路而实现的。更多还原

【Abstract】 Objective To investigate the effect of metastasis-associated in colon cancer 1(MACC1) on growth and migration of ovarian cancer cells.Methods Human ovarian cancer SKOV3 cells were divided into normal control group, negative control group and si-MACC1 group.After 48 h of transfection, cell proliferation was measured by MTT assay.The migration and apoptosis of cells were detected by flow cytometry and transwell assay, respectively.The expression of MACC1,PI3 K,p-Akt, PCNA and cleaved caspase 3 was determined by Western blot.Results Compared with normal or negative control group, the proliferation activity, migration ability and expression of MACC1,PI3 K,p-Akt and PCNA decreased, and the apoptosis rate and cleaved caspase 3 expression increased in si-MACC1 group(P<0.01 or P<0.05).Conclusion MACC1 gene silencing can inhibit the growth and migration of human ovarian cancer SKOV3 cells, and its mechanism may be involved in activation of PI3 K/AKT signaling pathway.更多还原