【摘要】 目的研究在重庆市地区血液病患者和健康人群中,IL-10基因启动子592位点基因多态性的分布情况及其与白血病易感性之间的关系。方法从医院住院部和体检中心分别选取各型白血病患者114例和健康体检者140例,应用PCR-限制性长度片段多态性方法,检测该研究人群中IL-10-592位点的多态性。结果 ALL组IL-10-592基因型频率分别为:纯合突变型(AA)8例(18.6%),杂合子(AC)5例(11.6%),野生型(CC)30例(69.8%);CLL组IL-10-592基因型频率分别为:纯合突变型(AA)11例(15.5%),杂合子(AC)10例(14.1%),野生型(CC)50例(70.4%);就对照组而言,其IL-10-592基因型的频率是80例纯合突变型(AA),占57.1%,46例杂合子(AC),占32.9%,14例野生型(CC),占10.0%。且统计数据显示,ALL和CLL两组基因型频率与对照组比较,差异具有统计学意义(P<0.001)。多元Logistic回归结果表明,与携带IL-10-592 A/A者相比较,携带IL-10-592 C/C者发生ALL的危险性为OR=26.82(95%CI:11.30～63.64,P=0.000)。携带IL-10-592 C/C基因型者患CLL的风险是携带IL-10-592 A/A基因型者的26倍(OR=26.70,95%CI:9.72～73.30,P=0.000)。结论研究结果提示IL-10-592位点基因多态性与急慢性淋巴白血病的易感性密切相关。更多还原

【Abstract】 Objective To discuss the polymorphisms of IL-10 gene and the association with susceptibility of leukemia in Han people in Chongqing. Methods ALL 43 patients and 71 patients with CLL, and 140 healthy controls were genotyped for IL-10-592 gene polymorphisms by the PCR-RFLP method. Results The results indicated that the frequencies of IL-10-592 genotypes in leukemia group was significantly different comparing control group(P < 0.001). Multivariate Logistic regression analysis showed that the risk of all in patients with il-10-592 C/C was 26.82(95% CI: 11.30～63.64, P=0.000) compared with those with il-10-592 A/A. The risk of CLL in patients with il-10-592 C/C genotype was 26 times higher than that in patients with il-10-592 A/A genotype(OR = 26.70, 95% CI: 9.72～73.30, P=0.000). Conclusion The results suggest that the polymorphism of IL-10-592 gene has close association with susceptibility of acute and chronic lymphatic leukemia.更多还原