【摘要】 为了获得蛹虫草CY1909菌株的最佳培养条件,通过单因素试验和响应面试验设计,对该菌株进行液态发酵培养基优化。得到生产虫草素的最优培养基配方:酵母蛋白胨52.25 g/L、酵母粉8.43 g/L、硫酸亚铁0.06 g/L、腺嘌呤3 g/L+甘氨酸12 g/L。在此条件下进行验证,测得虫草素产量6.35 g/L,与预测的产量几乎一致。优化后配方为高产虫草素及其后期开发应用提供了坚实的基础。更多还原

【Abstract】 In order to obtain the optimal culture conditions of Cordyceps militaris CY1909 strain, the liquid fermentation medium of the strain was optimized by the single factor test and response surface experiment design. The optimal medium for production of cordycepin formula was yeast peptone 52.25 g/L, yeast powder 8.43 g/L, ferrous sulfate 0.06 g/L, adenine+glycine(3+12) g/L. Under these conditions, the yield of cordycepin was 6.35 g/L, which was almost the same as the predicted yield. The optimized formula laid a foundation for high yield of cordycepin and its later development and application.更多还原