【摘要】 通过转录组比较分析筛选到6个水稻响应冷胁迫的基因,其中OsHI-XIP基因被重点研究。为了深入研究OsHI-XIP基因的生物学功能,从越光品种基因组中克隆了这个基因的序列,并构建了该基因的过量表达载体。通过农杆菌介导法将该载体导入赣香B株系的胚性愈伤组织中,获得了转基因阳性苗16株。本研究首次建成了赣香B的遗传转化体系,转化效率高达16.4%,明显高于一般籼稻品种的转化效率,为籼稻遗传转化及育种研究提供了技术支撑。更多还原

【Abstract】 Six rice genes responding to cold stress were screened through transcriptome comparative analysis, among which the OsHI-XIP gene was focused on. In order to further study the biological function of this gene, the OsHI-XIP gene sequence was cloned from the genome of Koshihikari variety, and an over-expression vector of the gene was constructed. The vector was introduced into the embryogenic callus of Ganxiang B strain by Agrobacterium-mediated method, and 16 transgenic positive seedlings were obtained. In the study, the genetic transformation system of Ganxiang B was established for the first time. The transformation efficiency was as high as 16.4%, which was significantly higher than that of general indica rice varieties. It provided technical support for indica rice genetic transformation and breeding research.更多还原