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基于正交试验的白桦扦插生根影响因素优化

Optimization of Factors Affecting Rooting of Betula platyphylla Cuttings Based on Orthogonal Test

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【作者】 李政纶; 陈坤; 姜静;

【Author】 LI Zheng-lun;CHEN Kun;JIANG Jing;State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University;

【通讯作者】 姜静;

【机构】 东北林业大学林木遗传育种国家重点实验室;

【摘要】 以4年生的白桦家系半木质化嫩枝为插条,采用3因素5水平正交试验设计,研究了激素种类、激素浓度、激素处理时间对白桦扦插成活的影响,以期为基层生产单位提供切实可行的白桦无性繁殖技术,白桦良种的规模化生产奠定基础。结果表明:3因素中只有激素浓度对扦插生根率、根鲜重、生根数量的影响达到显著水平(P<0.05);而激素种类对上述生根指标的影响均未达到显著水平。极差分析显示,影响生根率、生根数量的各因素排序:激素浓度对根鲜重的影响占主导地位,其次是激素处理时间,最后是激素种类;影响扦插生根率的各因素最适组合为20 mg/L IBA处理0 h;影响根鲜重的各因素最适组合为20 mg/L IBA处理2 h,影响生根数量的各因素最适组合为50 mg/L IBA处理时间为2 h。综合上述结果,确定影响白桦扦插生根指标的各因素最适组合:采用20~50 mg/L IBA处理白桦插穗0~2 h,即可获得最佳的扦插效果。

【Abstract】 The 4-year-old Betula platyphylla clone semi-lignified shoots were used as cuttings. The effects of hormone type, hormone concentration and hormone treatment time on the survival of Betula platyphylla cuttings were studied by three-factor and five-level orthogonal experiment design, so as to provide feasible asexual reproduction technology for Betula platyphylla production units at the basic level and lay the foundation for the large-scale production of Betula platyphylla varieties. The results showed that only hormone concentration had significant influence on rooting rate, fresh root weight and rooting quantity(P<0.05). The effects of hormone types on rooting indexes were not significant. The range analysis showed that the order of factors affecting rooting rate and quantity was as follows: the effect of hormone concentration on root fresh weight was dominant, followed by hormone treatment time, and the last was hormone type. The optimum combination of factors affecting rooting rate was IBA treatment of 20 mg/L for 0 h. The optimal combination of factors affecting root fresh weight was IBA treatment of 20 mg/L for 2 h and IBA treatment of 50 mg/L for 2 h. Based on the above results, the optimal combination of factors affecting the rooting index of Betula platyphylla cuttings was IBA of 20~50 mg/L for 0~2 h, and the optimal effect was obtained.

【基金】 国家重点研发计划课题资助(2017YFD0600603);林木遗传育种国家重点实验室创新项目(2020A01)
  • 【文献出处】 江西农业学报 ,Acta Agriculturae Jiangxi , 编辑部邮箱 ,2021年04期
  • 【分类号】S792.153
  • 【被引频次】5
  • 【下载频次】225
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