【摘要】 为了解扬州地区可生食蔬菜中大肠埃希菌的污染及其耐药情况,对2019年1—7月从扬州4家超市和2家农贸市场采集的492份胡萝卜、黄瓜、生菜和西红柿样品进行大肠埃希菌的分离鉴定,采用琼脂稀释法测定菌株对15种抗菌药物的最小抑菌浓度,并采用PCR及测序技术检测菌株携带重要耐药基因的情况。结果表明:492份蔬菜样品中共分离到68株大肠埃希菌,其中65株大肠埃希菌至少对一种抗菌药物耐药,对复方新诺明耐药率最高（73.53%）,对氨苄西林和四环素耐药率均高于50%,对磷霉素耐药率仅8.82%,对美罗培南、阿米卡星和多黏菌素全部敏感。floR（n=17,25.0%）检出率最高,其次为bla_CTX-M（n=8,11.76%）,包括bla_CTX-M-65、bla_CTX-M-55和bla_CTX-M-14,bla_CMY-2和fosA3检出率分别为7.35%（n=5）和8.82%（n=6）。这一研究提示2019年扬州地区部分可生食蔬菜中存在耐药大肠埃希菌的污染,应加强对蔬菜中耐药大肠埃希菌污染的监测。更多还原

【Abstract】 To understand the contamination and antimicrobial resistance of Escherichia coli in raw vegetables in Yangzhou. 492 vegetable samples including carrot, cucumber, lettuce, and tomato from four supermarkets and two markets in Yangzhou during January to July 2019 were collect to isolate E.coli. The E.coli isolates were tested for their antimicrobial susceptibility to 15 antimicrobial agents by the ager dilution method. PCR and sequencing were used to detect the presence of some important resistance genes in all strains. The results showed that a total of 68 E.coli isolates were obtained from 492 vegetable samples. Among them, 65 strains were resistant to at least one antimicrobial agent, with the highest resistance rate of sulfamethoxazole（73.53%）, the resistance rates of ampicillin and tetracycline were both higher than 50%, and the isolates showed the lowest resistance to fosfomycin（8.82%）. They were all sensitive to meropenem, amikacin and colistin. The detection rate of floR was the highest（n=17, 25.0%）, followed by bla_CTX-M（n=8, 11.76%）, including bla_CTX-M-65, bla_CTX-M-55 and bla_CTX-M-14. The detection rates of bla_CMY-2 and fosA3 were 7.35%（n=5） and 8.82%（n=6）, respectively. The study indicated that the contamination of antibiotic resistant E.coli has occurred in raw vegetables in some areas of Yangzhou in 2019, and the monitoring of antibiotic resistant E.coli in vegetables should be strengthened.更多还原