【摘要】 采用荧光定量PCR法,检测羧酸酯酶基因（CPIJ002786-RA）在淡色库蚊实验室敏感品系、抗性选育品系及4个现场种群（山东商河、山东孤岛、山东惠民、江苏江心洲）敏感株和抗性株中的相对表达水平。结果显示,幼虫浸渍法检测实验室敏感品系LC₅₀（半数致死浓度）0.03 mg/L,抗性选育品系LC₅₀为2.00 mg/L。WHO成蚊接触筒法（0.05%溴氰菊酯药膜）区分现场种群敏感株和抗性株,平均死亡率在48%～72%之间,敏感株平均击倒时间在32～52 min之间。淡色库蚊羧酸酯酶基因（CPIJ002786-RA）表达水平,实验室抗性选育品系是敏感品系的2.77倍（P<0.01）,山东商河、孤岛、惠民3个现场种群抗性株分别是敏感株的3.63（P<0.05）、5.67（P<0.05）和2.75倍（P<0.01）,江苏江心洲现场种群抗性株和敏感株无显著差异（P>0.05）。淡色库蚊羧酸酯酶基因（CPIJ002786-RA）高表达可能与溴氰菊酯抗药性密切相关,具有作为蚊抗药性现场检测靶标的潜在应用价值。更多还原

【Abstract】 Fluorescence quantitative PCR method was used to detect the relative expression levels of carboxylesterase gene（CPIJ002786-RA） in sensitive and resistant strains of Culex pipiens pallens of both laboratory population(LC₅₀=0.03 mg/L and 2.00 mg/L by larval bioassay), and sensitive and resistant strains（differentiated by WHO susceptibility tube bioassay） in 4 field populations（Shanghe/Gudao/Huimin of Shandong Province, Jiangxinzhou of Jiangsu Province）. The expression level of carboxylesterase（CPIJ002786-RA） was 2.77-fold higher in resistant strains than in susceptible strains of laboratory populations（P<0.01）, and were 3.65-, 5.67-and 2.75-fold higher in three resistant strains of field populations（Shanghe, Gudao and Huimin） than in susceptible strains（P<0.05）. However, the expression level of carboxylesterase was no significant difference between resistant and sensitive strains（P>0.05） in Jiangxinzhou field population. The over expression of carboxylesterase gene（CPIJ002786-RA） in Cx. pipiens pallens may be closely related to the deltamethrin resistance, which means a potential application value as an index gene in field resistance detection.更多还原