【摘要】 目的探讨大鼠肝再生中环状RNA(circ RNA)的表达变化以及对自噬的影响。方法以60只大鼠2/3肝切除0 h、2 h、6 h、12 h、24 h、30 h、36 h、72 h、120 h和168 h后的再生肝circRNAs、微小RNAs(miRNAs)、mRNA的高通量测序数据为基础,分析其在肝再生中的表达变化,进一步用miRanda aug 2010软件预测circRNAmiRNA和miRNA-mRNA靶标关系,并根据基因功能→miRNA功能→circRNA功能反向推测得到支配大鼠肝脏自噬的肝再生相关circRNAs和miRNAs,并构建它们的相互作用网络。结果与肝再生自噬相关的circRNAs、miRNAs、mRNA分别有102、42、153种。利用生物信息学分析,发现44种circRNAs、13种miRNAs及17种mRNA之间有相互作用关系,并以此构建了它们的共表达网络。结论筛选出了与肝再生自噬相关的circRNAs、miRNAs和mRNA,并构建了相互作用网络图谱。更多还原

【Abstract】 Objective Studies have shown that circular RNA( circRNA) is a molecular sponge of microRNA( miRNA) and can be used for the treatment and diagnosis of liver diseases. Therefore,this study aims to further investigate the expression profile of circRNA and its effect on autophagy in rat liver regeneration. Methods Based on the high-throughput sequencing in regenerated liver of 0 hour,2,6,12,24,30,36,72,120 and 168 hours after 2/3 hepatectomy in 60 rats,the expression profiles of circRNAs,miRNAs and mRNA were analyzed and their relationship were predicted by miRanda aug 2010 software. Furthermore,according to gene function → miRNA function → circRNA function,circRNAs and miRNAs that regulating autophagy were obtained in liver regeneration and compting endogenous( ceRNA) network was constructed. Results There were 102 circRNAs,42 miRNAs and 153 mRNAs associated with autophagy involved in liver regeneration. Using bioinformatics analysis,44 types of circRNAs,13 types of miRNAs and17 types of mRNA were found to interact with each other,and their co-expression network was constructed. Conclusion In this study,we screened out the circRNAs,miRNAs and mRNAs related to autophagy in liver regeneration and built the interaction network map.更多还原