【摘要】 目的探讨葛根素对去势脑缺血大鼠学习记忆功能的保护作用。方法将SD雌性大鼠随机分为正常对照组、OVX+2VO组、Sham-OVX+2VO组和葛根素低剂量组(25mg·kg^-1)、中剂量组(50mg·kg^-1)、高剂量组(100mg·kg^-1)。利用去卵巢（Ovariectomy,OVX）与双侧颈总动脉结扎（2-vessel occlusion,2-VO）结合的方法建立去势脑缺血（OVX+2VO）大鼠模型。给药组腹腔注射葛根素注射液,每天1次。跳台实验检测其学习记忆功能; TTC染色观察脑缺血情况; WST-1法和TBA法测定海马中超氧化物歧化酶（SOD）活力和丙二醛（MDA）含量;分光光度计和酶标仪分别检测乙酰胆碱酯酶（Ach E）活性和乙酰胆碱（Ach）含量。结果与OVX+2VO组比较,葛根素干预组脑缺血情况得到明显改善,SOD活力升高（P<0.05）,MDA含量降低（P<0.05）,Ach E活性减弱（P<0.05）,Ach含量升高（P<0.05）,跳台实验潜伏期延长（P<0.05）、错误次数减少（P<0.05）。结论葛根素能改善去势脑缺血大鼠的学习记忆功能,其作用机制可能与降低Ach E活性、增加Ach和MDA含量、降低SOD活力有关。更多还原

【Abstract】 Objective To investigate the protective effect of puerarin on learning and memory function in ovariectomized rats with chronic cerebral ischemia.Methods SD rats were randomly divided into control group,model group,low( 25 mg·kg^-1),medium( 50 mg · kg^-1) and high( 100 mg · kg^-1) dose of puerarins group. The model of ovariectomy（ OVX） with the bilateral common carotidartery ligation（ 2 VO） were established.Puerarin injection was given intraperitoneally once a day.Learning and memory function was measured by Step-down test.Cerebral ischemia was observed by TTC staining.The activity of SOD and MDA in hippocampus were determined by WST-1 and TBA methods. The activity of Ach E and content of Ach were measured by spectrophotometer and microplate reader separately.Results Compared with OVX+2 VO group,the cerebral ischemia was significantly relieved,the activity of SOD was higher（ P<0.05）,the content of MDA and the activity of Ach Ewere weakened（ P<0.05）,the content of Ach was elevated（ P<0.05）.Step down latency was prolonged（ P<0.05） and the numbers of errors was fewer（ P<0.05）.Conclusion Puerarin can improve the learning and memory function of ovariectomized rats with cerebral ischemia,which may be related to decrease the activity of Ach E,increase the content of MDA and reduce SOD activity.更多还原