【摘要】 采用内转录间隔区(internal transcription spacer, ITS)序列分析和形态学鉴定相结合的方法,从腐烂的植物根中分离得到霉菌,利用真菌通用引物扩增菌株rDNA ITS区序列并对其进行鉴定。将序列提交至GenBank中以确定物种分类信息,进行物种同源序列比对分析,并通过Figtree构建物种进化系统发育树。ITS分子测序以及形态学鉴定结果表明,菌株LJM-001隶属于木霉菌属(Trichoderma)。同时,利用该菌株作为指示微生物,模拟重金属和抗生素复合污染的生存环境,并以霉菌的干重、还原型谷胱甘肽含量以及蛋白质含量为表征,分析复合污染下微生物的生理生化变化,并对其进行相关性分析。实验结果表明,抗生素胁迫下的重金属抗性变化主要为协同杀菌和协同抗性,重金属离子浓度和种类均对菌株LJM-001耐抗生素性能有显著影响。更多还原

【Abstract】 The molds isolated from rotting plant roots was identified by the method of combining internal transcription spacer(ITS) sequence and morphological identification, and the rDNA ITS region of the strain was amplified with fungal universal primers.Then the sequence was submitted to GenBank to obtain the species classification information, and compared with homologous sequence of the species to construct the species evolution phylogenetic tree with Figtree.The results of ITS molecular sequencing and morphological identification showed that the strain LJM-001 belongs to Trichoderma koningiopsis.At the same time, the strain LJM-001 as an indicator microorganism was used to living environment of compound with heavy metals and antibiotics, and explore the strain’s adapting performance characterized by dry weight of mold, GSH content and protein content to analyzing the physiological and biochemical changes of microorganisms under compound pollution and analyzing their correlations.The results showed that the strain’s response in heavy metal resistance under antibiotic stress were mainly synergistic bactericidal and synergistic resistance, and indicated that the concentration and type of heavy metal both had a significant impact on the antibiotic resistance of strain LJM-001.更多还原