【摘要】 目的:探讨自噬相关蛋白p62在喉癌组织中的表达及临床意义。方法:选取2010年1月～2015年4月于福建省肿瘤医院行喉癌根治性切除术的115例患者为研究对象,采用免疫组化检测喉癌组织及癌旁正常组织石蜡标本p62蛋白表达水平,并分析与临床病理特征及预后的关系。采用实时荧光定量PCR(RT-PCR)和蛋白质印迹法(Western Blot)检测25例冷冻保存的新鲜喉癌组织标本中p62mRNA及蛋白的表达水平。结果:石蜡标本中,p62在喉癌石蜡组织中表达阳性率为56.5%,高于癌旁正常组织的23.5%。p62与喉癌的浸润深度、淋巴结转移、病理分期有关,p62阳性患者的5年生存率低于p62阴性患者,差异有统计学意义(P<0.05)。在新鲜喉癌组织中,p62mRNA及蛋白表达水平比相应的癌旁正常组织增高,差异有统计学意义(P<0.05)。结论:喉癌组织中p62的表达水平增高,与喉癌的发生、发展有关,阳性表达预示患者预后欠佳。更多还原

【Abstract】 Objective To investigate the expression and clinical significance of autophagy related protein p62 in laryngeal carcinoma.Method 115 patients who underwent radical resection of laryngeal carcinoma in Fujian Provincial Cancer Hospital from January 2010 to April 2015 were selected as research objects. Immunohistochemistry was used to detect the expression level of p62 protein in paraffin specimens of laryngeal carcinoma and adjacent tissues, and the relationship with clinicopathological characteristics and prognosis was analyzed.Real-time fluorescence quantitative PCR(RT-PCR) and Western Blot were used to detect the expression levels of p62 mRNA and protein in 25 freeze-preserved fresh laryngeal cancer tissues.Results The positive rate of p62 expression in paraffin tissues of laryngeal carcinoma was 56.5%, higher than 23.5% in adjacent tissues.p62 was related to the infiltration depth, lymph node metastasis and pathological stage of laryngeal cancer, and the 5-year survival rate of p62 positive patients was lower than that of p62 negative patients(P<0.05).The expression levels of p62 mRNA and protein in fresh laryngeal carcinoma tissues were higher than those in corresponding paracancer tissues(P<0.05).Conclusion The increased expression of p62 in laryngeal cancer is related to the occurrence and development of laryngeal cancer, and indicates poor prognosis.更多还原