【摘要】 为研究北极地区海洋藻类可培养附生细菌的多样性,利用稀释平板法自2019年7月采集的北极新奥尔松地区的海洋红藻多管藻(Polysiphonia sp.)样品中分离纯化得到60株可培养的藻类附生细菌。16S rRNA基因序列测定分析表明所分离的藻类附生细菌主要隶属于γ-变形菌纲(39株,65%),其次隶属于放线菌纲(10株, 17%)和黄杆菌纲(8株, 13%),仅有少量隶属于α-变形菌纲(2株, 3%)和芽孢杆菌纲(1株, 2%),其中的优势细菌属为Psychrobacter属(22株, 37%)和Pseudoalteromonas属(12株, 20%)。此次获得了较多极地藻类多管藻的附生细菌菌株,初步揭示了多管藻可培养附生细菌的多样性,为多管藻自身以及其附生细菌生态功能及应用开发研究奠定了基础。更多还原

【Abstract】 To analyze the diversity of epiphytic bacteria in Arctic algae, a red algae sample(Polysiphonia sp.) was collected from Ny-?lesund, Svaldbard, Arctic in July 2019. A total of 60 cultivable strains of epiphytic bacteria were isolated from the sample using the dilution plating method. Analysis of 16 S rRNA gene sequences revealed that the isolated strains mainly belonged to the class γ-Proteobacteria(39 strains, 65% of the total strains isolated from the red algae samples), followed by the classes Actinobacteria(10 strains, 17%), Flavobacteriia(eight strains, 13%), α-Proteobacteria(two strains, 3%) and Bacilli(one strain, 2%). The strains were affiliated with 12 genera with the predominant genera being Psychrobacter(22 strains, 37%) and Pseudoalteromonas(12 strains, 20%). The results preliminarily illustrate the diversity of cultivable epiphytic bacteria in Arctic red algae(Polysiphonia sp.), laying a foundation for studies on the ecological functions and exploitation of these particular Arctic microbial resources.更多还原