【摘要】 为研究Chi-miR-3031对奶山羊乳腺上皮细胞增殖和凋亡的影响,利用Chi-miR-3031 mimic、inhibitor转染西农萨能奶山羊乳腺上皮细胞。采用CCK-8、RT-qPCR、Western blot等试验方法,研究了Chi-miR-3031对体外培养的奶山羊乳腺上皮细胞增殖的影响和凋亡的调控机制。结果显示,与NC组相比,Chi-miR-3031 mimic显著提高了PCNA、C-MYC mRNA的表达量(P<0.05)和Bcl-2/Bax比值(P<0.01),并且显著抑制了Caspase3的蛋白表达(P<0.01)。研究结果表明,Chi-miR-3031能促进奶山羊乳腺上皮细胞的增殖,抑制细胞凋亡。更多还原

【Abstract】 In order to explore the effects of Chi-miR-3031 on the proliferation and apoptosis of mammary epithelial cells of dairy goats, the mammary gland epithelial cells of Xinong Saanen dairy goat were transfected with Chi-miR-3031 mimic and inhibitor. CCK-8, RT-qPCR and Western blot were used to research the effects of Chi-miR-3031 on the regulation mechanism of mammary epithelial cell proliferation and apoptosis in vitro. Compared with NC group, Chi-miR-3031 mimic significantly increased the expression of PCNA and C-MYC mRNA(P<0.05) and the ratio of Bcl-2/Bax(P<0.05) and significantly inhibited the expression of CASPASE3 protein(P<0.01), which indicates that Chi-miR-3031 can promote the proliferation and inhibit the apoptosis of mammary epithelial cells.更多还原