【摘要】 目的研究分析CD4+CD25+Foxp3+调节性T细胞(Treg)与再生障碍性贫血(AA)的相关性,探讨Treg细胞在AA中的诊断意义。方法方便选取2017年1月—2019年1月期间该院血液科收治的初发AA患者40例,根据发病时情况分为急性再生障碍性贫血组(SAA组,22例)和慢性再生障碍性贫血组(CAA组18例),正常成年人为对照组(CN组,20例)。采用流式细胞术检测Treg细胞的表达率(%);并用荧光定量PCR法检测其外周血中Foxp3 mRNA的表达水平。结果流式细胞检测显示SAA组及CAA组血清样本CD4+、CD4+CD25+和Foxp3Treg细胞比率与CN组相比均显著下降,差异有统计学意义(P<0.05);另与CAA组(2.64±0.48)%相比,SAA组在Foxp3Treg细胞百分比(1.51±0.34)%,差异有统计学意义(P<0.05)。相关基因表达显示相对于CN组,CAA组及SAA组患者外周血中的Foxp3mRNA均呈低表达,差异有统计学意义(P<0.05),且SAA组明显低于CAA组,差异有统计学意义(P<0.05)。结论再生障碍性贫血(CAA和SAA)在发病时均伴有明显的CD4+CD25+Foxp3+调节性T细胞表达率下降,且SAA组下降明显低于CAA组,说明CD4+CD25+Foxp3+Treg细胞比率能够作为一项指标来评估AA的严重程度。更多还原

【Abstract】 Objective To study and analyze the correlation between CD4 +CD25 +Foxp3 + regulatory T cells(Treg) and aplastic anemia(AA), and to explore the diagnostic significance of Treg cells in AA. Methods It is convenient to select 40 patients with first-onset AA admitted to the Department of Hematology in the hospital from January 2017 to January 2019,and are divided into acute aplastic anemia group(SAA group, 22 cases) and chronic aplastic anemia according to their onset conditions Group(18 case s in CAA group), normal adults served as control group(20 cases in CN group). The expression rate(%) of Treg cells was detected by flow cytometry; and the expression level of Foxp3 mRNA in the peripheral blood was detected by fluorescence quantitative PCR. Results Flow cytometry showed that the ratios of CD4 +, CD4 +CD25 + and Foxp3 Treg cells in serum samples of SAA group and CAA group were significantly decreased compared with CN group,the difference was statistically significant(P<0.05); in addition, compared with CAA group(2.64±0.48)%, there was a difference in the percentage of Foxp3 Treg cells(1.51±0.34)% between the SAA group,the difference was statistically significant(P<0.05). The expression of related genes showed that Foxp3 mRNA in peripheral blood of CAA group and SAA group were lower than that of CN group(P<0.05), and the SAA group was significantly lower than the CAA group,the difference was statistically significant(P<0.05). Conclusion Aplastic anemia(CAA and SAA) is accompanied by a significant decrease in the expression rate of CD4+CD25+Foxp3+ regulatory T cells, and the decrease in the SAA group is significantly lower than that in the CAA group, indicating that CD4+CD25+Foxp3+Treg cells ratio can be used as an indicator to assess the severity of AA.更多还原