【摘要】 以工业下脚料大豆胚芽为原料,经烘干、粉碎、过筛后,以乙醇为溶剂水浴振荡提取胚芽中的黄酮,对提取时间、乙醇浓度、提取温度和液料比进行单因素试验,并通过4因素3水平响应面分析实验优化大豆胚芽中黄酮的提取工艺.结果表明:提取时间、乙醇体积分数、提取温度和液料比对黄酮的提取率有一定的影响,各因素的影响次序为:液料比>提取时间>乙醇体积分数>提取温度.最佳提取条件为温度46.8℃,时间60 min,液料比50,乙醇体积分数60%,在此工艺条件下,提取率为80.11%,与理论预测值基本吻合.更多还原

【Abstract】 Using industrial scraps of soybean germ as raw material,after drying,crushing,and sieving,the flavonoids in the germ were extracted by shaking in a water bath with ethanol as a solvent.Single factor tests were carried out on the extraction time,ethanol concentration,extraction temperature and liquid-to-material ratio.The extraction process of flavonoids from soybean germ was optimized by response surface experiments with 4 factors,3 levels experiment.The results showed that:extraction time,ethanol concentration,extraction temperature and liquid-to-material ratio had certain effects on the extraction rate of flavonoids.The order of influence of the factors were:liquid-to-material ratio>extraction time>ethanol concentration>extraction temperature.The optimal extraction conditions were 46.8 ℃,60 min,liquid-solid ratio 50,ethanol concentration 60%.Under this technological condition,the extraction rate was 80.11%,which was basically consistent with the theoretical prediction value.更多还原