【摘要】 目的:探讨莪术油对人子宫内膜癌HEC-1-B细胞增殖、凋亡及Caspase-3、Bax、Bcl-2蛋白表达的影响。方法:应用莪术油各浓度(0、120、240、480、960 mg·L^-1)处理HEC-1-B细胞48 h后,细胞的增殖抑制率用MTT法检测;莪术油对HEC-1-B细胞凋亡应用Hoechest33258染色法进行观察;Western blot法检测莪术油对HEC-1-B细胞Caspase-3、Bax与Bcl-2蛋白表达水平。结果:与对照组相比较,莪术油能够明显抑制HEC-1-B细胞的增殖,且呈一定的浓度依赖性;Hoechest33258染色可见明显的细胞凋亡形态学改变;莪术油作用HEC-1-B细胞48 h后,与对照组比较,Caspase-3、Bax蛋白表达明显增加、Bcl-2蛋白表达明显下降。结论:莪术油能明显抑制HEC-1-B细胞增殖,诱导细胞凋亡,其机制可能与上调Caspase-3和Bax蛋白表达、下调Bcl-2蛋白表达相关。更多还原

【Abstract】 Objective: To explore into the effects of zedoary turmeric oil on proliferation and apoptosis and expressions of Caspase-3 and Bax and Bcl-2 in HEC-1-B.Methods: After HEC-1-B cells were treated with zedoary turmeric oil at different concentrations(0,120,240,480,960 mg·L^-1) for 48 hours.The inhibition rate of cell proliferation was detected by MTT method.Hoechst 33258 staining was used to observe the effect of zedoary turmeric oil on the apoptosis of HEC-1-B cells.Western blot was used to detect the expressions of Caspase-3,Bax and Bcl-2 in HEC-1-B cells.Results: Compared with the control group, zedoary turmeric oil could significantly inhibit the proliferation of HEC-1-B cells in a concentration dependent manner.Hoechst 33258 staining showed obvious morphological changes of apoptosis.After having been treated with zedoary turmeric oil for 48 hours, compared with the control group, the expressions of Caspase-3 and Bax protein increased significantly, while the expression of Bcl-2 protein decreased significantly.Conclusion: Zedoary turmeric oil can significantly inhibit the proliferation of HEC-1-B cells and induce apoptosis.The mechanism may be related to up regulating the expressions of Caspase-3 and Bax protein and down-regulating the expression of Bcl-2 protein.更多还原