【摘要】 目的:利用网络药理学探讨前痛定方治疗慢性前列腺炎(CP)的潜在作用机制。方法:从中药系统药理学分析平台(TCMSP)和中药化学数据库(Neosuite TCMID)收集前痛定方中的有效成分并预测作用靶点,与Genecards、OMIM、DrugBank网站所收集的CP潜在靶点进行相互交集后,筛选出前痛定方治疗CP的潜在靶点,然后利用Cytoscape分析软件(v3.7.2)及其插件Cyto NCA绘制"中药-有效成分-靶点"网络图并分析其核心成分,同时利用R语言进行GO分子功能和KEGG通路富集分析,最后在蛋白质互作网络分析平台STRING数据库构建靶蛋白质互作网络并分析其核心靶点。结果:前痛定方治疗CP的潜在靶点共124个,其核心成分为川陈皮素、黄柏酮酸、黄柏内酯、诺米林、壬基醇-10,核心靶点为磷脂酰肌醇-3-激酶催化亚基α、鸡肉瘤基因、丝裂原活化蛋白激酶1、Harvery鼠肉瘤基因、丝裂原活化蛋白激酶8等,相关通路涉及生长、增殖、血管生成、炎症、免疫防御和耐药性等,其中磷脂酰肌醇3激酶、白细胞介素-17、丝裂原活化蛋白激酶途径可能是其治疗的关键通路。结论:前痛定方治疗CP的作用机制具有多成分、多靶点、多通路的特点,为今后进一步探析前痛定方有效成分与体内靶点之间的作用机制提供了新的思路和理论基础。更多还原

【Abstract】 Objective: To investigate the potential mechanism of action of Qiantongding prescription in the treatment of chronic prostatitis(CP) based on network pharmacology.Methods: The TCMSP and Neosuite TCMID databases were used to collect the effective constituents of Qiantongding prescription and predict their action targets, which were intersected with the potential targets of CP collected by Genecards, OMIM,and DrugBank websites to screen out the potential targets of Qiantongding prescription in the treatment of CP.Cytoscape 3.7.2 and its plug-in Cyto NCA were used to plot the “traditional Chinese medicine-effective constituent-target” network and analyze the core constituents, and then R language was used to perform GO molecular function analysis and KEGG pathway enrichment analysis.Finally, STRING database, an analysis platform for protein-protein interaction network, was used to construct the protein-protein interaction network and analyze core targets.Results: Qiantongding prescription had 124 potential targets in the treatment CP,with the core constituents including nobiletin, keto acid, obaculactone, nomilin, and nonanol-10 and the score targets including phosphatidylinositol 3-kinase catalytic subunit α,chicken sarcoma gene, mitogen-activated protein kinase 1,Harvery rat sarcoma virus gene, and mitogen-activated protein kinase 8.Related pathways were involved in growth, proliferation, angiogenesis, inflammation, immune defense, and drug resistance, and among these pathways, the phosphatidylinositol 3-kinase, interleukin-17,and mitogen-activated protein kinase pathways might be the key pathways for treatment.Conclusion: Qiantongding prescription has the features of multiple constituents, targets, and pathways in the treatment of CP,which provides new ideas and a theoretical basis for further research on the interaction between the effective constituents of Qiantongding prescription and related targets in body.更多还原