【摘要】 目的:基于NF-κB/MAPK信号通路研究鱼藤素对过敏性哮喘（allergic asthma, AA）大鼠的作用机制。方法:雄性Wistar大鼠腹腔注射卵清蛋白（ovalbumin, OVA）致敏液[100μg OVA+1 mg Al（OH）₃]加雾化OVA制备慢性哮喘大鼠模型,将模型制备成功的大鼠随机分为模型组、鱼藤素（低、中、高剂量）组(1 mg·kg^-1、2 mg·kg^-1、4 mg·kg^-1),每组8只,另取8只大鼠作为正常组,腹腔注射相应的药物7 d。检测大鼠的肺功能;ELISA法检测大鼠肺泡灌洗液中白细胞介素-1β（interleukin-1β,IL-1β）、IL-13水平;HE染色观察大鼠肺组织病理变化;Western Blot法检测大鼠肺组织p-NF-κB p65、NF-κB p65、p-p38 MAPK、p38 MAPK、p-ERK、ERK蛋白表达。结果:与模型组比较,鱼藤素低、中、高剂量组大鼠呼吸速率显著降低（P<0.05）,吸气峰流量、呼气峰流量显著升高（P<0.05）;大鼠肺泡灌洗液中IL-1β、IL-13水平显著降低（P<0.05）;大鼠肺组织p-NF-κB p65/NF-κB p65、p-p38 MAPK/p38 MAPK、p-ERK/ERK水平显著降低（P<0.05）;肺组织病理损伤有所改善。结论:鱼藤素可改善AA大鼠的肺功能及气道炎症反应,可能与抑制NF-κB/MAPK通路有关。更多还原

【Abstract】 Objective: Based on the NF-κB/MAPK pathway to study the mechanism of action of deguelin on allergic asthma（AA） rats.Methods: Male Wistar rats were injected intraperitoneally with aerosolized ovalbumin（OVA） sensitizer [100 μg OVA+1 mg Al（OH）₃] and aerosolized OVA to prepare chronic asthma models.Rats successfully modeled were randomly divided into a model group, deguelin low(1 mg·kg^-1),medium(2 mg·kg^-1),and high(4 mg·kg^-1) dose groups, 8 rats in each group, and another 8 rats as the normal group.The corresponding drugs were injected intraperitoneally for 7 days.The lung function of the rats was detected.The interleukin-1β（IL-1β） and interleukin-13（IL-13） levels in rat alveolar lavage fluid were detected by ELISA method, and expression of p-NF-κB p65,NF-κB p65,p-p38 MAPK,p38 MAPK,p-ERK,ERK protein in rat lung tissue were detected by Western Blot Method.Results: Compared with the model group, rats in the low, medium, and high dose groups of deguelin showed a significant decrease in respiratory rate（P<0.05）,and a significant increase in peak inspiratory flow and peak expiratory flow（P<0.05）.The levels of IL-1β and IL-13 in the lavage fluid were significantly reduced（P<0.05）.And the levels of p-NF-κB p65/NF-κB p65,p-p38 MAPK/p38 MAPK,and p-ERK/ERK in rat lung tissue Significantly decreased（P<0.05）.At the same time, the pathological damage of lung tissue was alleviated.Conclusion: Deguelin can improve lung function and airway inflammation in AA rats, which may be related to the inhibition of the NF-κB/MAPK signaling pathway.更多还原