【摘要】 目的:研究昆布多糖（laminarin, LA）对柯萨奇病毒B3（coxsackievirus group B type3,CVB3）诱导的新生小鼠病毒性心肌炎的调节作用。方法:将100只BALB/c新生小鼠随机分为对照组（n=20）、LA组（n=20）、CVB3组（n=30）和CVB3+LA组（n=30）。CVB3组和CVB3+LA组腹腔注射CVB3进行模型复制,造模后LA组和CVB3+LA组腹腔注射LA(50 mg·kg^-1)干预,连续注射12 d。检测新生小鼠心脏功能,血清诱导型一氧化氮合酶（iNOS）和白细胞介素-10（interleukin-10,IL-10）、肌红蛋白（myohemoglobin, Mb）、肌酸激酶同工酶-MB（creatine kinase-MB,CK-MB）和心肌肌钙蛋白I（cardiac troponin I,cTn I）水平;HE染色观察心肌组织病理变化;TUNEL法检测心肌细胞凋亡;Western Blot法检测心肌组织IL-6、Bax、Bcl-2、NF-κB p65、p-p65、TNF-α表达;RT-PCR法检测心肌组织CVB3 mRNA和IFN-γ mRNA的表达。结果:与CVB3组相比,CVB3+LA组新生小鼠尾部动脉收缩压（systolic blood pressure, SBP）、心率（heart rate, HR）和左心室收缩压（left ventricular systolic pressure, LVSP）指数明显升高（P<0.01）,Mb、CK-MB、cTn l、iNOS水平明显下降（P<0.01）,心肌细胞凋亡率显著下降（P<0.01）,IL-10、Bcl-2和IFN-γ mRNA的表达显著升高（P<0.01）,CVB3 mRNA的水平及p-p65、TNF-α、IL-6和Bax蛋白表达显著下降（P<0.01）,同时可改善心肌组织损伤。结论:LA可能通过抑制炎症反应和细胞凋亡保护CVB3诱导病毒性心肌炎新生小鼠的心脏功能。更多还原

【Abstract】 Objective: To explore the regulation effects of laminarin（LA） in inhibiting coxsackievirus group B type3（CVB3） induced viral myocarditis in neonatal mice.Methods: A total of 100 BALB/c neonatal mice were randomly divided into control group（n=20）,LA group（n=20）,CVB3 group（n=30） and CVB3+LA group（n=30）.CVB3 group and CVB3+LA group were intraperitoneally injected to copy the model.After molding, the LA group and CVB3+LA group were intraperitoneally injected with 50 mg·kg^-1 laminarin for 12 d.Cardiac function, levels of iNOS and IL-10,Mb, CK-MB,and cTn I in serum, and protein expression levels of Bax/Bcl-2,NF-KB p65,p-p65,and TNF-α in myocardial tissues were measured in each group.HE staining and TUNEL staining were used to analyze the pathological features of myocardial tissues.Results: Compared with CVB3 group, the MAP,HR,and LVSP indexes were significantly increased in CVB3+LA group（P<0.01）,and the expression levels of serum Mb, CK-MB,cTn l, iNOS were significantly decreased（P<0.01）,and the apoptosis rate of cardiomyocytes was significantly decreased（P<0.01）,the expression of IL-10,Bcl-2 and IFN-γ mRNA was significantly increased（P<0.01）,while the CVB3 mRNA,p-p65,TNF-α,IL-6 and Bax protein were significantly down-regulated（P<0.01）,meantime, it improves the pathological damage of myocardial tissue.Conclusion: LA may improve the cardiac function of CVB3-induced viral myocarditis in neonatal mice by inhibiting inflammation and apoptosis.更多还原