【摘要】 目的:观察中风1号方对急性脑出血（acute intracerebral hemorrhage,AICH）模型大鼠的影响,探讨其初步机制。方法:采用自体尾部血液注入法复制大鼠急性脑出血模型。将大鼠随机分为假手术组、模型组、中风1号方低剂量(6.75 g·kg^-1)组和中风1号方高剂量(13.5 g·kg^-1)组,连续给予相应的药物灌胃3天。用Longa 5级评分法评估神经功能损伤程度;计算脑组织含水量;检测脑组织伊文思蓝（evans blue,EB）含量,评价血脑屏障通透性;ELISA法检测脑出血周围组织肿瘤坏死因子α（tumor necrosis factor,TNF-α）、白细胞介素1β（interleukin-1β,IL-1β）水平。结果:与假手术组比较,模型组大鼠24 h、72 h的Longa 5级评分,脑组织含水量,EB含量,TNF-α、IL-1β水平均升高（P<0.05,P<0.01）。与模型组比较,中风1号方低剂量可降低模型大鼠神经行为学评分（72 h）,脑组织含水量（72 h）,EB含量,TNF-α、IL-1β水平（72 h）（P<0.05,P<0.01）;中风1号方高剂量组可降低模型大鼠的神经行为学评分（72 h）,脑组织含水量（72 h）,EB含量（24 h、72 h）,TNF-α、IL-1β水平（24 h、72 h）（P<0.01）。结论:中风1号方能改善急性脑出血模型大鼠神经功能损伤,减轻脑水肿,缓解血脑屏障受损,其机制可能与降低脑组织TNF-α、IL-1β水平有关。更多还原

【Abstract】 Objective:To observe the effect of Zhongfeng No.1 Prescription on acute intracerebral hemorrhage（AICH） model rats and explore its preliminary mechanism.Methods:The model of acute cerebral hemorrhage in rats was replicated by autologous tail blood injection.The rats were randomly divided into sham operation group,model group,Zhongfeng No.1 Prescription low-dose(6.75 g·kg^-1) group and Zhongfeng No.1 Prescription high-dose(13.5 g·kg^-1) group,and given corresponding drugs continuously 3 days.The Longa 5-level scores method was used to evaluate the degree of neurological damage;calculate the water content of brain tissue;detect the content of evans blue（EB） in brain tissue to evaluate the permeability of the blood-brain barrier;the ELISA method was used to detect the levels of tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β） in tissues surrounding cerebral hemorrhage.Results:Compared with the sham operation group,the Longa 5-level scores,brain tissue water content,EB content,TNF-α,IL-1β levels of the model group increased at 24 h and 72 h（P<0.05,P<0.01）;compared with the model group,the low dose of Zhongfeng No. 1 prescription can reduce the water content of brain tissue（72 h）,EB content,TNF-α,IL-1β levels（72 h）（P<0.05,P<0.01）; the high-dose group of Zhongfeng No.1 prescription can reduce the neurological function score（72 h）,brain tissue water content（72 h）,EB content（24 h,72 h）,TNF-α,IL of model rats-1β level（24 h,72 h）（P<0.01）.Conclusion:Zhongfeng No.1 Prescription can improve the nerve function damage of acute cerebral hemorrhage model rats,reduce brain edema,and relieve blood-brain barrier damage.The mechanism may be related to the reduction of brain tissue TNF-α and IL-1β levels.更多还原