【摘要】 目的:研究健脾消癌方含药血清对HCT116细胞迁移、侵袭的影响,并初步探讨其机制。方法:10%、15%、20%的健脾消癌方含药血清处理HCT116细胞,划痕实验、Transwell侵袭实验检测细胞迁移及侵袭能力,Western blot检测细胞内基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)的表达。结果:处理24 h、48 h,与相同浓度鼠血清对照组相比,10%、15%、20%的含药血清组划痕距离均增宽(P<0.05,P<0.01);与相同浓度鼠血清对照组相比,10%、15%、20%的含药血清组的迁移抑制率及侵袭抑制率均明显增大(P<0.05),均与浓度正相关。与15%的鼠血清对照组比较,15%的含药血清组MMP-2、MMP-9蛋白表达显著下调(P<0.01)。结论:健脾消癌方抗复发转移机制可能是通过下调MMP-2、MMP-9蛋白表达,从而抑制HCT116细胞迁移、侵袭。更多还原

【Abstract】 Objective:To study the effect of Jianpi Xiaoai Prescription drug containing serum on the migration and invasion of HCT116 cells,and to explore its mechanism.Methods:HCT116 cells were treated with 10%,15% and 20% Jianpi Xiaoai Prescription drug containing serum.Cell migration and invasion were detected by scratch test and Transwell invasion test.The expression of matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9) were detected by Western blot.Results:After 24 h and 48 h treatment,the scratch distance of 10%,15%,20% drug containing serum group was wider than that of the control group(P<0.05,P<0.01);compared with the control group,the migration inhibition rate and invasion inhibition rate of 10%,15%,20% drug containing serum group were significantly increased(P<0.05),and were positively correlated with the concentration;compared with the control group,the expression of MMP-2 and MMP-9 in the 15% drug containing serum group was significantly decreased(P<0.01).Conclusion:The anti recurrence and metastasis mechanism of Jianpi Xiaoai Prescription may be through down regulating the expression of MMP-2 and MMP-9,thus inhibiting the migration and invasion of HCT116 cells.更多还原