【摘要】 目的:观察益气活血法对局灶性脑缺血再灌注大鼠的脑保护作用,并探讨其可能作用机制。方法:建立局灶性脑缺血再灌注大鼠模型,建模成功大鼠随机分为模型组(n=17)、血塞通组(n=17)、补阳还五汤组(n=17)、联合组(n=18),另设假手术组(n=17)和空白组(n=17)。各组大鼠给予相应干预。比较各组大鼠1、3、7 d神经缺损评分、脑组织含水量、脑梗死体积百分比,检测脑组织无翅型MMTV整合位点家族成员3a(Wnt3a)、糖原合成酶激酶-3β(GSK-3β)、β-连环蛋白(β-catenin)、细胞周期蛋白D1(CyclinD1)、B淋巴细胞瘤-2基因(Bcl-2)、Bax的mRNA及蛋白表达情况。结果:与模型组比较,血塞通组、补阳还五汤组和联合组大鼠1、3、7 d神经缺损评分、大脑组织含水量、脑梗死体积百分比、脑组织GSK-3βmRNA及蛋白相对表达量均降低(P<0.05),脑组织Wnt3a、β-catenin、CyclinD1的mRNA及蛋白相对表达量,以及Bcl-2/Bax均升高(P<0.05);联合组上述指标改善情况均优于血塞通组和补阳还五汤组(P<0.05)。结论:益气活血法对局灶性脑缺血再灌注大鼠具有脑保护作用,其机制与激活Wnt3a/β-catenin信号通路有关。更多还原

【Abstract】 Objective: To observe the protective effect of the method of Yiqi Huoxue on the brain of rats with focal cerebral ischemia and reperfusion, and explore its possible mechanism. Methods: A rat model of focal cerebral ischemia-reperfusion was established. The rats were randomly divided into model group(n=17), Xuesaitong(血塞通) group(n=17), Buyang Huanwu Decoction(补阳还五汤) group(n=17), combined group(n=17), sham operation group(n=15) and blank group(n=15). Rats in each group were given corresponding intervention. The neurological deficit scores, brain tissue water content, and cerebral infarction volume percentage were compared in each group of rats at 1, 3, and 7 days, and wingless-type MMTV integration site family member 3 a(Wnt3 a) and glycogen synthase kinase-3β(GSK-3β), β-catenin(β-catenin), Cyclin D1(Cyclin D1), B lymphoma-2 gene(Bcl-2),Bax m RNA and protein expression. Results: Compared with the model group, the 1, 3, and 7 d neurological deficit scores, brain tissue water content, cerebral infarction volume percentage, brain tissue GSK-3β mRNA and protein expression levels were all decreased(P<0.05), and the expression levels of brain tissue Wnt3a, β-catenin,Cyclin D1 mRNA and protein, and the levels of Bcl-2/Bax were all increased in the Xuesaitong group, Buyang Huanwu Decoction group and the combination group(P<0.05). The improvement of the above indicators in the combined group was greater than that of the Xuesaitong group and Buyanghuanwutang group(P<0.05). Conclusion:The method of Yiqi Huoxue has brain protection in rats with focal cerebral ischemia and reperfusion, and its mechanism is related to the activation of Wnt3a/β-catenin signaling pathway.更多还原