【摘要】 目的:探讨通关藤提取物(MTE)对人肝癌HepG2细胞凋亡的影响及诱导凋亡的途径。方法:采用不同浓度MTE(15、20、30 mg/mL)处理HepG2细胞,AnnexinV-FITC/PI双染法检测MTE对HepG2细胞凋亡的影响,JC-1染色法检测HepG2细胞线粒体膜电位,蛋白质印迹(Western blotting)技术检测HepG2细胞线粒体凋亡途径相关蛋白的表达变化。结果:不同浓度MTE可诱导HepG2细胞出现细胞凋亡,并不同程度降低HepG2细胞线粒体跨膜电位。MTE可下调B细胞白血病/淋巴瘤-2(Bcl-2)蛋白的表达,同时上调Bcl-2相关X蛋白(Bax)、细胞色素c(Cytochromec)、半胱氨酸蛋白酶9(Caspase-9)和Caspase-3的蛋白表达。结论:MTE可通过诱导HepG2细胞凋亡发挥抗肿瘤作用,其作用机制与内源性线粒体凋亡途径有关。更多还原

【Abstract】 Objective: To investigate the effect of extract of Tongguanteng(Marsdenia tenacissima)(MTE) on the apoptosis of human hepatocarcinoma HepG2 cells and the way of inducing apoptosis. Methods: HepG2 cells were treated with different concentrations of MTE(15, 20, 30 mg/mL). Annexin V-FITC/PI double staining method was used to detect the effect of MTE on HepG2 cell apoptosis. JC-1 staining method was used to detect mitochondrial membrane potential of HepG2 cells. Western blotting was used to detect the expression of mitochondrial apoptosis pathway related proteins in HepG2 cells. Results: Different concentrations of MTE could induce apoptosis and decrease mitochondrial transmembrane potential in HepG2 cells. MTE can down-regulate the protein expression of Bcl-2, and up-regulate the expression of Bax, cytochrome c, Caspase-9 and Caspase-3.Conclusion: MTE can exert an anti-tumor effect by inducing HepG2 cell apoptosis, and its mechanism of action is related to the endogenous mitochondrial apoptosis pathway.更多还原