【摘要】 目的分析揭阳市2018—2020年食物中毒细菌性病原菌的检测情况,为食源性疾病主动监测和制定细菌性食物中毒的预防控制策略提供科学依据。方法对2018—2020年揭阳市疾控中心的疑似食物中毒样本进行细菌性病原菌检测,并对2020年检出的12株沙门菌挑选8株菌株进行全基因组测序,分析其耐药基因的携带情况。对检测结果进行整理分析。结果 2018—2020年共检测疑似食物中毒样本70份,总阳性率为70.00%(49/70)。致病菌主要为副溶血性弧菌,占48.57%,其次为沙门菌,占22.85%,检出混合感染(副溶血性弧菌+沙门菌)5株,占7.14%,检出产气荚膜梭菌2株,占2.85%,金黄色葡萄球菌和蜡样芽孢杆菌均检出1株,占1.41%。肛拭子检出率最高(85.71%)。食物中毒主要发生在第2季度。沙门菌血清型主要为鼠伤寒沙门菌和韦太夫雷登沙门菌,多位点序列分型为ST19和ST365型。鼠伤寒沙门菌携带多种耐药基因。结论揭阳市食物中毒致病菌的检出率较高,主要是副溶血性弧菌、鼠伤寒沙门菌、韦太夫雷登沙门菌引起的,并存在两种细菌混合感染的情况,食物中毒主要发生在第2季度。鼠伤寒沙门菌耐药情况较严重,有关部门应加强食品卫生安全监管,根据食物中毒的特点,完善食物中毒预警机制,警惕耐药菌株在本地引起食源性中毒的风险。更多还原

【Abstract】 Objective Analysis of the results of food poisoning bacterial pathogens in Jieyang City from2018 to 2020, provide scientific basis for active and formulation of prevention which control strategies for bacterial food poisoning.Methods The samples of suspected food poisoning in Jieyang City Center for Disease Control and Prevention from 2018 to 2020 were tested for bacterial pathogens, and select 8 strains of salmonella detected in 2020 for whole genome sequencing, analyzed the carrying status of their dug resistance genes, organized and analyzed test results.Results From 2018 to 2020, 70 cases of suspected food poisoning were tested, and the total positive rate was 70.00%(49/70). The commonpathogens included Vibrioparahaemolyticus(48.57%), Salmonella(22.58%),mix-infection(7.14%), clostridium perfringens(2.85%), staphylococcus aureus and bacillus cereus(1.41%). Anal swabs detected the highest rate(85.71%). The salmonella serotypes were mainly salmonella typhimurium and salmonella vetafreden. The MLST types were ST19 and ST365. Salmonella typhimurium carried multiple drug resistance genes.Conclusion The detection rate of food poisoning pathogens in this city is high, mainly caused by vibrio parahaemolyticus, salmonella typhimurium, salmonella Witifreden, and there is a mixed infection of bacteria. Food poisoning mainly occurred in season 2. Salmonella typhimurium drug situation is very serious. Relevant departments should strengthen food hygiene and safety supervision, improve the food poisoning early warning mechanism based on the characteristics of food poisoning, and be alert to the risk of food-borne poisoning caused by drug-resistant strains in the local area.更多还原