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内生菌株4-3培养条件的优化(英文)

Optimization of the Culture Conditions for the Endophytic strain 4-3

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【作者】 龚平陈娴李咏梅曾艳唐冰璇

【Author】 GONG Ping;CHEN Xian;LI Yong-mei;ZENG Yan;TANG Bing-xuan;JIANG Hai-yan;LIU Dong-hua;WU Shan-dong;Hunan Institute of Microbiology;

【通讯作者】 陈娴;

【机构】 蒋海燕刘冬华伍善东湖南省微生物研究院

【摘要】 解淀粉芽孢杆菌4-3是一株性能优良的植物内生菌,可用作良好的微生态制剂原材料。为优化该菌株的培养条件,以内生菌4-3的活菌数为评价指标,采用单因素试验对其液体发酵的培养条件进行了优化。优化后的培养条件为:培养温度32℃,初始pH值7.2,培养转速220 r/min,接种量3%,培养56 h,该条件下可获得较高浓度的菌体。

【Abstract】 Bacillus amyloliquefaciens 4-3 is an excellent plant endophyte that can be used as a good raw material for microecological preparations. For this reason,it is important to optimize the culture conditions of this strain. The number of viable bacteria of strain 4-3 in this study was the evaluation index, and the culture conditions of the liquid fermentation were optimized by single factor experiment. The optimized culture condition was as follows: incubating temperature 32°C, initial pH value 7.2, rotation speed 220 r/min, inoculum concentration 3% and incubating 56 h,under which higher concentrations of Bacillus amyloliquefaciens could be obtained.

【关键词】 内生菌培养条件液体发酵
【Key words】 Plant endophyteCulture conditionLiquid fermentation
【基金】 Supported by Science and Technology Plan Project of Hunan Province (2015NK3056)~~
  • 【文献出处】 Agricultural Science & Technology ,农业科学与技术(英文版) , 编辑部邮箱 ,2021年04期
  • 【分类号】Q93-335
  • 【下载频次】30
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