【摘要】 为探明重金属镉对感染了细菌的农田蜘蛛免疫防疫功能的影响，以拟水狼蛛为研究对象，对镉处理组和对照组蜘蛛均进行染菌处理（S. aureus和E. coli），检测染菌6 h和24 h后各处理蜘蛛体内能量物质和免疫解毒酶系的变化。研究结果显示：在感染S. aureus6 h和24 h以及感染E. coli 6 h后，镉处理组拟水狼蛛体内可溶性糖和可溶性蛋白含量明显增加；与对照组相比，染E. coli 24 h和染S. aureus 6 h后镉处理组蜘蛛体内的乙酰胆碱酯酶(AChE)活性均显著下降，这表明镉抑制了染菌拟水狼蛛体内AChE的活性；染E.coli 6 h和染S. aureus 24 h后镉处理组蜘蛛的PPO活性较对照组显著升高，说明镉诱导了染菌拟水狼蛛体内PPO的活性。这些结果表明镉能影响染菌拟水狼蛛体内能量物质含量和免疫解毒酶活性。更多还原

【Abstract】 In order to investigate the effects of cadmium on the immune defense function of spiders infected with bacteria, Pirata subpiraticus was taken as research object, and the cadmium-treated group and the control group were all infected with bacteria(S. aureus and E. coli). After 6 and 24 h of infection, the energy substances contents and the activity of immune detoxification enzyme system in Pirata subpiraticus were determined. The results showed that after 6 and 24 h of infection with S. aureus and 6 h of infection with E. coli, the amount of soluble sugars and soluble proteins of Pirata subpiraticus in the cadmium-treated group increased significantly. Compared with the control, after 6 h of infection with S. aureus and 24 h of infection with E. coli, the activity of acetylcholinesterase(AChE) of Pirata subpiraticus in the cadmium-treated group decreased significantly. This indicated that cadmium inhibited the activity of AChE in infected Pirata subpiraticus. After 24 h of infection with S. aureus and 6 h of infection with E. coli, the activity of polyphenol oxidase(PPO) was signicantly higher than that of the control, indicating that cadmium induced the activity of PPO in infected Pirata subpiraticus. These results suggested that cadmium can affect the content of energy substance and immune detoxification enzyme activity in infected Pirata subpiraticus.更多还原