【摘要】 解淀粉芽孢杆菌4-3是一株性能优良的植物内生菌,可用作良好的微生态制剂原材料。为优化该菌株的培养条件,以内生菌4-3的活菌数为评价指标,采用单因素试验对其液体发酵的培养条件进行了优化。优化后的培养条件为:培养温度32℃,初始pH值7.2,培养转速220 r/min,接种量3%,培养56 h,该条件下可获得较高浓度的菌体。更多还原

【Abstract】 Bacillus amyloliquefaciens 4-3 is an excellent plant endophyte that can be used as a good raw material for microecological preparations.Therefore,it is important to optimize the culture conditions of this strain.The number of viable bacteria of strain 4-3 in this study was the evaluation index,and the culture conditions of the liquid fermentation were optimized by single factor experiment.The optimized culture conditions were:incubating temperature 32℃,initial pH 7.2,rotation speed 220 r/min,inoculum concentration 3% and incubating 56 h,under which higher concentrations of Bacillus amyloliquefaciens could be obtained.更多还原